Species authentication of commercial beef jerky based on PCR-RFLP analysis of the mitochondrial 12S rRNA gene  

作　　者：Shi-Yi Chen Yi-Ping Liu Yong-Gang Yao 

机构地区：[1]Key Laboratory of Animal Models and Human D&ease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China [2]Research Institute of Animal Genetics and Breeding, Siehuan Agricultural University, Ya'an 625014, China

出　　处：《Journal of Genetics and Genomics》2010年第11期763-769,共7页遗传学报（英文版）

基　　金：supported by the National High-tech R&D Program (863 Program)(No.2008AA101001);the Ministry of Agriculture of China (No.2009ZX08009159B);Yunnan Province (No.2009CI119)

摘　　要：In this study, we determined species-specific variations by analyzing the mitochondrial 12S rRNA gene sequence variation （-440 bp） in 17 newly obtained sequences and 90 published cattle, yak, buffalo, goat, and pig sequences, which represent 62 breeds and 17 geo- graphic regions. Based on the defined species-specific variations, two endonucleases, Alu I and Bfa I, were selected for species authentication using raw meat/tissue samples and the PCR-RFLP method. Goat and pig were identified using the Alu I enzyme, while cattle, yak, and buffalo were identified by digestion with Bfa I. Our approach had relatively high detection sensitivity of cattle DNA in mixed cattle and yak products, with the lowest detectable threshold equaling 20% of cattle DNA in a mixed cattle/yak sample. This method was successfully used to type commercial beef jerky products, which were produced by different companies utilizing various processing technologies. Our results show that several yak jerky products might be implicated in commercial fraud by using cattle meat instead of yak meat.In this study, we determined species-specific variations by analyzing the mitochondrial 12S rRNA gene sequence variation （-440 bp） in 17 newly obtained sequences and 90 published cattle, yak, buffalo, goat, and pig sequences, which represent 62 breeds and 17 geo- graphic regions. Based on the defined species-specific variations, two endonucleases, Alu I and Bfa I, were selected for species authentication using raw meat/tissue samples and the PCR-RFLP method. Goat and pig were identified using the Alu I enzyme, while cattle, yak, and buffalo were identified by digestion with Bfa I. Our approach had relatively high detection sensitivity of cattle DNA in mixed cattle and yak products, with the lowest detectable threshold equaling 20% of cattle DNA in a mixed cattle/yak sample. This method was successfully used to type commercial beef jerky products, which were produced by different companies utilizing various processing technologies. Our results show that several yak jerky products might be implicated in commercial fraud by using cattle meat instead of yak meat.

关 键 词：12S rRNA gene PCR-RFLP meat species identification beefjerky commercial fraud 

分 类 号：S511.035.1[农业科学—作物学] Q959.482[生物学—动物学]