用于生物检测的链霉亲和素修饰γ-Fe_2O_3@Au复合颗粒的制备与表征  

Fabrication and Characterization of Streptavidin γ-Fe_2O_3@Au Nanocomposites for Biological Application

作　　者：刘丽赏[1] 刘洪娜[2] 李松[1,2] 邓燕[1] 李小龙[1] 何农跃[1,2] 

机构地区：[1]湖南工业大学绿色包装与生物纳米技术应用湖南省重点实验室,株洲412008 [2]东南大学生物电子国家重点实验室,南京210096

出　　处：《化学学报》2010年第20期2041-2046,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.60801007;60927001;60971045);863国家高技术研究发展计划(Nos.2007AA022007);国家重点基础研究发展计划-纳米研究重大科学研究计划(Nos.2007CB936104;2010CB933903);中国博士后科学基金(No.20100471362);国家科技重大专项"十一五"计划科研支撑平台(No.2009ZX10004-311);湖南省自然科学基金(Nos.09JJ3017;09JJ4004);湖南省教育厅科研基金(No.08B018);苏州市科技计划(No.YJS0949)资助项目

摘　　要：制备了粒径为30nm左右的γ-Fe2O3磁性颗粒,利用巯基硅烷(MPTES)的偶联作用最终制备了粒径约为35.9nm的金磁复合颗粒.用X射线粉末衍射仪(XRD)、透射电子显微镜(TEM)、紫外可见吸收光谱(UV-Vis)、傅里叶变换红外光谱(FT-IR)、振动样品磁强计(VSM)等方法对所得金磁颗粒的表面形貌、大小、结构、光学和磁学性质进行了表征.结果表明:金成功地包覆到了γ-Fe2O3颗粒的表面,所得金磁颗粒具有超顺磁性.利用静电吸附作用,链霉亲和素有效修饰到了γ-Fe2O3@Au复合磁性颗粒表面.通过扫描仪检测其捕捉Cy3标记寡核苷酸序列后的荧光信号,证明了链霉亲和素修饰的γ-Fe2O3@Au复合磁性颗粒有生物活性,且没有荧光背景,在生物检测领域表现出了很大的应用前景.γ-Fe2O3 nanoparticles about 30 nm were prepared using chemical co-precipitation firstly,and then they are modified with thiol group by means of 3-mercaptopropyltriethoxysilane （MPTES）,γ-Fe2O3@Au nanoparticles with size of 35.9 nm were obtained.XRD,TEM,UV,FT-IR and VSM analysis showed the surface appearance,size,structure,optical and magnetics property of the gold coated magnetic nanoparticles （γ-Fe2O3@Au）.The result showed that Au was coated on the surface of γ-Fe2O3 successfully,the obtained γ-Fe2O3@Au composite nanoparticles are superparamagnetic,streptavidin was modified on the surface of γ-Fe2O3@Au nanoparticles effectively.The fluorescent signal before and after adsorption of Cy3 labeled oligonucleotide showed that the streptavidin modified γ-Fe2O3@Au nanoparticles had good biological activity and without fluorescence background.Most kinds of biological molecular can be combined on the surface of the streptavidin modified γ-Fe2O3@Au nanoparticles through biotin-streptavidin system.Being able to be separated with magnetic field easily,they have great potential application prospects.

关 键 词：MPTES(3-Merca ptopropyltriethoxysilane) γ-Fe2O3@Au复合颗粒 链霉亲和素 生物检测 生物电子学 

分 类 号：Q503[生物学—生物化学]