溶菌酶在羊毛表面的静电自组装固定化及其抗菌性能  

Immobilization of Lysozyme on Wool Surface via Layer-by-layer Electrostatic Self-assembly and Its Antibacterial Property

作　　者：王强[1] 高笑笑[1] 范雪荣[1] 袁久刚[1] 

机构地区：[1]江南大学生态纺织教育部重点实验室,无锡214122

出　　处：《化学学报》2010年第20期2099-2103,共5页Acta Chimica Sinica

基　　金：国家863计划(No.2008AA02Z203)资助项目

摘　　要：通过层层静电自组装法将溶菌酶和聚苯乙烯磺酸钠(PSS)交替沉积在羊毛纤维表面,赋予其抗菌性.应用扫描电镜(SEM)考察组装后纤维表面形态,Zeta电位、染色、溶菌酶活性测试研究纤维表面组装机制并考察改性羊毛抗菌效果.SEM结果表明组装后纤维表面粗糙,部分凹槽被填埋,证明溶菌酶/PSS自发组装到纤维表面.表面电位、染料上染量、溶菌酶活力随纤维表面沉积物质不同,均呈现明显的"层层交替振荡"现象,证实了组装后纤维表面电荷的交替变化;织物紫外线透过率随组装层数增加呈近似线性降低,表明溶菌酶在羊毛表面实现了多层组装,揭示了羊毛纤维表面抗菌功能涂层的层层静电自组装构筑机制.同时,组装层数增多,溶菌酶活性逐渐增大.载酶羊毛抗菌率达到91.7%,具有良好的抗菌性能.Layer-by-layer electrostatic self-assembly deposition of lysozyme and poly（sodium 4-styrene-sulfonate） （PSS） on wool fibers which endowed them with antibacterial properties were studied.Scanning electron microscopy （SEM） presented the surface morphologies of assembled wool.Zeta potentials,dyeing,activity of lysozymes revealed the mechanism of layer-by-layer electrostatic self-assembly process on fibers and the antibacterial effects were determined.SEM showed rougher surfaces of modified wool fibers with partially-filled grooves,indicating that lysozyme and PSS were assembled on the surfaces of wool fibers.The electrical potential,dye uptake,activity of lysozyme all showed obvious ＂layer-by-layer alternative oscillation＂,which demonstrated the changes of electrical charges on the surfaces of assembled fibers;an approximately linear decrease in UV transmissivity with the increasing assembled layers indicated that lysozymes were multilayeredly assembled on the surface of wool,these revealed the fabrication mechanism of layer-by-layer electrostatic self-assembly antibacterial coatings on fibers surfaces.Meanwhile,the activity of lysozyme was gradually increasing with the increase in the layer numbers of assembled enzyme.The antimicrobial ratio of assembled wool could reach 91.7%,indicating a good antimicrobial property.

关 键 词：溶菌酶 固定化 静电自组装 抗菌 羊毛 

分 类 号：Q814[生物学—生物工程]