具抗凝血作用的铁、铜三元配合物与人血清白蛋白的相互作用  

Interaction of the Ternary Complexes with Anticoagulant Property of Iron,Copper with Human Serum Albumin

作　　者：宋玉民[1] 刘哲[1] 王坤杰[1] 栾妮娜[1] 

机构地区：[1]西北师范大学化学化工学院,兰州730070

出　　处：《化学学报》2010年第21期2191-2198,共8页Acta Chimica Sinica

基　　金：甘肃省自然科学基金(No.0710JRZA108);甘肃省高分子材料重点实验室资助项目

摘　　要：采用光度法研究了具抗凝血作用的过渡金属铁和铜的华法灵、水杨酸三元配合物与人血清白蛋白的相互作用,观察到铁、铜三元配合物使人血清白蛋白荧光产生猝灭现象,猝灭方式为静态猝灭,并计算了配合物与人血清白蛋白的结合常数和结合位点数.根据不同温度下的热力学函数,确定了配合物与人血清白蛋白的作用力类型.并且发现三元配合物的存在明显改变人血清白蛋白的构象.并讨论了配合物使人血清白蛋白构象发生变化的可能原因.The interaction between human serum albumin and transition metals iron complexes of warfarinanion salicylic acid ternary complexes with anti-cruor function has been studied by spectrophotometry.It was observed that transition complexes of iron and copper can reduce the fluorescence intensity of human serum albumin.The way of fluorescence quenching was static quenching,and then calculated the binding constant and binding sites.The style of action of human serum albumin with complexes was gained according to the thermodynamic functions at different temperatures.And found that iron and copper complexes have obviously changed the conformation of human serum albumin.The possible reason for which the complex makes serum albumin conformational changes is discussed.

关 键 词：铁、铜华法灵、水杨酸三元配合物 人血清白蛋白 荧光光谱 紫外吸收光谱 圆二色谱 

分 类 号：R96[医药卫生—药理学]