数字全息显微术应用于生物样品相衬成像的实验研究  

Experimental Study on the Quantitative Phase-Contrast Imaging of the Biological Samples by Digital Holographic Microscopy

作　　者：赵洁[1] 王大勇[1] 李艳[1] 王云新[1] 张亦卓[1] 

机构地区：[1]北京工业大学应用数理学院,北京100124

出　　处：《中国激光》2010年第11期2906-2911,共6页Chinese Journal of Lasers

基　　金：北京市教育委员会科技发展计划重点项目;北京市自然科学基金重点项目(B类)(KZ200910005001);北京市属市管高等学校人才强教计划资助课题

摘　　要：构建了无透镜傅里叶变换数字全息实验系统,实现了对一组生物样品特别是活体细胞的高保真度定量相衬成像。系统的简单性在于其数值再现过程只需直接对全息图进行一次快速傅里叶变换,而对由此引入到相位像中的畸变,则采用两步相减法予以校正。成像物体首先选用家蚊口器和洋葱表皮细胞的生物教学样片,进而使用无标记的活体宫颈癌细胞。实验中,不仅观察到了静态生物样品清晰的形态,而且观察到活体细胞形态的动态变化,充分表明了数字全息显微术应用于生物样品相衬成像的有效性。A setup of the lensless Fourier transform digital holography is built to get the quantitative high-fidelity phase-contrast images of the biological samples.The basic advantage of such system is that the numerical reconstruction is directly applying only once fast Fourier transform to the intensity distribution of the hologram.However,the aberration appears in the phase image obtained by this kind of reconstruction algorithm and degrades the quality of the phase image seriously.Then,two-step subtraction method is applied to correct the phase aberration.In the experiments,at first two biological sample-plates for teaching are used as the objects,which are the mouse of the skeeter and the epidermal cells of the onion respectively.Then the living free-of-marker cervical carvinoma cells are presented as the object.Not only the clear morphologies of the static biological samples,but also the dynamic variations of the living cells are observed.The experimental results verify the validity of the digital holographic microscopy for the quantitative phase-contrast imaging of the biological samples.

关 键 词：全息 数字全息 相衬成像 生物样品 活体细胞 相位畸变校正 两步相减法 

分 类 号：O438.1[机械工程—光学工程]