胆囊癌的傅里叶变换红外光谱研究  

Fourier Transform Infrared Sqectroscopic Study on Gallbladder Carcinoma

作　　者：孙启刚[1] 刘亚奇[1] 徐怡庄[2] 张元福[2] 吴瑾光[2] 周孝思[1] 徐智[1] 凌晓锋[1] 

机构地区：[1]北京大学第三医院普外科,北京100191 [2]北京大学化学与分子工程学院,北京100871

出　　处：《光谱学与光谱分析》2010年第12期3195-3198,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50673005);北京市自然科学基金项目(2072020)资助

摘　　要：运用衰减全反射(attenuated total reflection,ATR)探头与傅里叶变换红外(Fourier transform infra-red,FTIR)光谱仪,测定并分析了新鲜离体的胆囊癌组织18例和良性组织139例的FTIR光谱。结果表明:(1)胆囊癌的1 167和1 123 cm-1谱带的峰位显著地向低波数移动(P<0.05),而1 309 cm-1谱带的峰位显著地向高波数移动(P<0.05)。(2)胆囊癌组织光谱多个峰的相对强度I2 856/I1 461,I1 167/I1 461,I1 123/I1 461和I1 082/I1 461明显升高(P<0.05)。(3)1 167和1 082 cm-1谱带的半高宽显著升高(P<0.05),谱带1 461 cm-1的半高宽则显著降低(P<0.05)。(4)癌组织1 750 cm-1谱带的出现几率明显增加(P<0.05)。与胆囊良性组织相比较,癌组织光谱中与脂类、糖和核酸相关的谱带均发生了明显的变化。Fourier transform infrared（FTIR） spectroscopy with an attenuated total reflection（ATR）probe was used to study the benign and malignant tissues of gallbladder.FTIR spectra of 18 tissues of gallbladder carcinoma and 139 benign tissues were collected and analyzed.The results demonstrated that bands related to lipid,carbohydrate,and nucleic acid from malignant samples were significantly different from those of benign ones：（1） The peak positions of 1 167 and 1 123 cm-1 shifted toward lower wave number（P〈0.05） but that of 1 309 cm-1 to the higher one（P0.05）;（2） The relative intensity ratios of I2 856/I1 461,I1 167/I1 461,I1 123/I1 461,I1 082/I1 461 increased significantly（P〈0.05）（3）The half-widths of 1 167 and 1 082 cm-1increased significantly（P〈0.05）,whereas the half-width of 1 461 cm-1 decreased significantly（P〈0.05）.（4） The probability of 1 750 cm-1 band of gallbladder carcinoma tissues increased significantly（P〈0.05）.

关 键 词：胆囊癌 慢性胆囊炎 FTIR光谱 

分 类 号：O657.3[理学—分析化学]