益气养阴、消癥通络中药对早期糖尿病大鼠肾组织Nephrin基因表达的影响  

Influence of Chinese herbal remedies on renal nephrin gene expression in rats with early diabetes

作　　者：张江华[1] 陈志强[1] 孙玉凤[2] 赵雯红[1] 王月华[3] 裴国超[1] 刘晓冉[1] 

机构地区：[1]河北医科大学中西医结合研究所,河北050011 [2]河北医科大学附属第二医院消化科 [3]河北医科大学中医院肾内科

出　　处：《北京中医药大学学报》2010年第2期113-116,140,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：河北省自然科学基金资助项目(No.C2008001074);河北省卫生厅资助项目(No.08165)

摘　　要：目的探讨益气养阴、消癥通络中药对早期糖尿病(DM)大鼠肾组织nephrin mRNA及肾脏超微结构的影响。方法将SD大鼠随机分为6组:肾切组、模型组、厄贝沙坦组及中药低、中、高剂量组,按各组相应药物剂量灌胃。于第6周末检测各组大鼠24 h尿蛋白(UPro)定量及肾功能水平;取肾脏行HE染色以及透射电镜观察肾皮质组织病理改变;RT-PCR法测肾皮质nephrin表达水平。结果模型组大鼠与肾切组大鼠相比,光镜及电镜下已出现糖尿病肾病病理改变,各治疗组肾皮质病理病变较轻,厄贝沙坦组、中药低、中剂量组与模型组、高剂量组比较Upro量显著下降(P<0.05);治疗组与模型组比较肾功能明显改善(P<0.05);厄贝沙坦组及中药低、中、高剂量组与模型组比较nephrin mRNA表达水平显著上调(P<0.05)。结论益气养阴、消癥通络中药对链脲佐菌素(STZ)诱导的DN大鼠肾脏具有一定的保护作用。Objective To investigate the influences of Chinese herbal remedies（CHR） with the actions of replenishing qi and nourishing yin,and resolving mass and freeing the collateral vessels on renal nephrin-mRNA and ultrastructure in rats with early diabetes.Methods All SD rats were randomly divided into 6 groups including group 1（with nephrectomy）,group 2（model）,group 3（with irbesatan）,group 4（with low-dose CHR）,group 5（with mid-dose CHR） and group 6（with high-dose CHR）.The relevant drugs or CHR were given intragastrically to different groups respectively.At the end of 6th week the quantity of 24-hour urine protein（UPro） and kidney function were detected.The pathological changes of renal cortex were observed by using transmission electron microscope（TEM） after HE staining.The expression of nephrin was determined by applying RT-PCR.Results There were pathological changes of diabetic nephropathy observed by the light microscope and TEM in group 2 and 1,which were alleviated in other 4 groups.The quantity of UPro decreased significantly in group 3,4 and 5 compared with that in group 2 and 6（P0.05）.The renal function was improved significantly in all treatment groups compared with that in group 2（P0.05）.In group 3,4,5 and 6 the expression of nephrin-mRNA increased significantly compared with that in group 2（P0.05）.Conclusion CHR with the actions of replenishing qi and nourishing yin,and resolving mass and freeing the collateral vessels have a protective effect on kidney in rats with diabetic nephropathy induced by STZ.

关 键 词：益气养阴 消癥通络 糖尿病大鼠 超微结构 NEPHRIN 

分 类 号：R285.5[医药卫生—中药学]