四氯化碳致小鼠急性肝损伤模型的研究  

Studies of Hepatotoxicity Model of Mice Induced by Carbon Tetrachloride

作　　者：娄猛猛[1] 李国玉[2] 王航宇[2] 马庆东[2] 高建波[2] 王金辉[2] 

机构地区：[1]石河子大学药学院 [2]石河子大学药学院新疆特种植物药资源省部共建教育部重点实验室,新疆石河子832002

出　　处：《农垦医学》2010年第3期196-198,共3页Journal of Nongken Medicine

基　　金：国家重大新药创制项目(2010ZX09401-304)

摘　　要：目的:通过追踪观察模型小鼠肝损伤程度,筛选四氯化碳(CC l4)致昆明种小鼠急性肝损伤模型的最佳剂量。方法:采用随机对照分组的方法,对各模型组的昆明种小鼠给予不同剂量的CC l4,进行腹腔注射,检测血清ALT、AST水平,并观察肝组织病理变化。结果:与正常组相比,CC l4高浓度组(0.3%)和中浓度组(0.2%)显著升高血清中ALT和AST的活力(P<0.05)。结论:0.2%10m l/kg为CC l4致昆明种小鼠急性肝损伤模型较好的剂量。Objective：The purpose of this study was to optimize carbon tetrachloride-induced hepatotoxicity model in Kunming mice with respect to dose.Methods：Different dosages of carbon tetrachloride were given to Kunming mice by abdominal injection.Serum ALT,AST were analyzed.Moreover,the morphological change of mice liver tissue were observed by routine pathology technique（HE） after administration CCl4.Results：Compared with the control group,the levels of serum ALT and AST in 0.3% and 0.2% groups increased significantly（p0.05）.Conclusion：0.2% 10ml/kg was the best dose of carbon tetrachloride-induced hepatotoxicity model in Kunming mice.

关 键 词：四氯化碳 急性肝损伤 小鼠 

分 类 号：R34[医药卫生—基础医学]