真空渗透法转化植物的研究  

IN PLANTA TRANSFERMATION BY VACCUM INFILTRATION

作　　者：张钰[1,2] Adrian J.Calter 

机构地区：[1]哈尔滨医科大学生物及医学遗传学教研室 [2]加拿大国家研究院植物生物技术研究所

出　　处：《生物技术》1999年第2期35-36,共2页Biotechnology

摘　　要：以拟南芥为材料介绍一种不需组培的原位植物转化方法—真空渗透法。将含有Ｔ—ＤＮＡ载体的农杆菌细胞悬液，用真空渗透的方式转化愈伤的完整植株，从而直接获得转化的种子。该法快速简便、重复性好，不需经过组培阶段即可获得转化植株。Adrian J. Culter (Plant Biotechnology Institute, National Research Council of Canada, Canada)A method for in situ transformation, vaccum infiltration is presented. The approach is vaccum infiltration of a suspension of Agrobacterium cells containing T-DNA vector into Arabidopsis thaliana; thereby the transformed seeds can be harvested directly. This reproducible, non-tissue cultural procedure yields stably transformed A. thaliana seeds and its transformation frequency is high enough for introducing foreign genes and characterizing their expression.

关 键 词：真空渗透 转化 拟南芥 植物 

分 类 号：Q785[生物学—分子生物学]