光系统II核心天线CP43和CP47的分离纯化及光谱性质研究  

PURIFICATION AND SPECTRAL CHARACTERISTICS OF CP43 AND CP47 FROM PSⅡCORE COMPLEX IN SPINACH(SPINCIA OLERACEA) LEAVES

作　　者：单际修[1] 杨昆云[1] 李良璧[1] 匡廷云[1] 王居硕[2] 赵南明[2] 

机构地区：[1]中国科学院植物研究所光合作用研究中心 [2]清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室

出　　处：《生物物理学报》1999年第1期144-151,共8页Acta Biophysica Sinica

基　　金："九五"国家自然科学基金重大项目

摘　　要：采用ＤＥＡＥ－ＦｒａｃｔｏｇｅｌＴＳＫ６５０Ｓ阴离子交换树脂柱层析法从菠菜（ＳｐｉｎａｃｉａＯｌｅｒａｃｅａ）中分离纯化了ＰＳＩＩ核心天线复合物ＣＰ４３和ＣＰ４７，计算出每分子ＣＰ４３含１９－２０个Ｃｈｌａ和４－５个β－Ｃａｒ；每分子ＣＰ４７含２０－２１个Ｃｈｌａ和３－４个β－Ｃａｒ。普通及三维（３Ｄ）低温（７７Ｋ）荧光发射光谱的测定证明，纯化的ＣＰ４３和ＣＰ４７都具有较好的完整性。常温（２９８Ｋ）吸收光谱的测定，证明纯化的ＣＰ４３和ＣＰ４７的红光区最大吸收峰分别位于６７１ｎｍ和６７４ｎｍ，但是它们在该区的四阶导数光谱均给出６７０ｎｍ和６８２ｎｍ两个峰，这表明，它们均至少含有两种不同结合状态的Ｃｈｌａ分子。PSII core antenna CP43 & CP47 were purified from spinach(Spinica oleracea) by DEAE-Fractogel TSK 650S anion-exchange chromatography. It was obtained by calculating that each molecule of CP43 contents 19-20 chla and 4-5 β-carotene and each molecule of CP47 contents 20-21 chla and 3-4 β carotene. By measurement of their normal and 3-dimension (3D) low temperature (77k) fluorescence emission spectra, it was proved that the purified antenna core complexes CP43 and CP47 were both in higher integrity. The normal temperature (298k) absorption spectra of purified CP43 and CP47 show that they have two maximum absorption peaks at 671nm (CP43) and 674nm(CP47) in red region respectively. However, the fourth derivatives of these two absorption spectra in that region both gave two peaks at 670nm and 682nm. From these results it is pointed out that there are at least two kinds of binding state of chla bownd to CP43 and CP47. Possible mechanism of energy transfer in CP43 and CP47 are also discussed.

关 键 词：光系统Ⅱ 核心天线 CP43 CP47 吸收光谱 

分 类 号：Q945.11[生物学—植物学] O657.3[理学—分析化学]