四种方法检测伯氏疏螺旋体IgG抗体比较  

Comparative Study on Four Methods for Detecting Serum Antibody against Borrelia Burgdorferi

作　　者：赵文明[1] 刘振龙[1] 沈海中[1] 

机构地区：[1]首都医科大学微生物学教研室

出　　处：《上海实验动物科学》1999年第1期29-30,共2页Shanghai Laboratory Animal Science

摘　　要：用Ｄｏｔ－ＥＬＩＳＡ法、ＥＬＩＳＡ法、ＩＦＡ法及ＣＦ法进行了检测兔莱姆病血清ＩｇＧ抗体的比较实验。对１０例人工感染伯氏疏螺旋体（莱姆病病原体）的兔血清的检测结果表明，Ｄｏｔ－ＥＬＩＳＡ法与ＥＬＩＳＡ法，ＩＦＡ法及ＣＦ法的符合率分别为１００％、９０％和７０％；对１２例正常兔血清的检测，符合率均为１００％。这提示，Ｄｏｔ－ＥＬＩＳＡ法可能成为一种用于检测人或动物莱姆病血清抗体的简便方法。Serum IgG antibody from rabbits artificially infected with Borrelia burgdorferi was detected with new-established Dot-ELISA,routine ELISA,IFA and CF,respectively.Ten serum samples from infected rabbits were examined,the results of Dot-ELISA showed 100%,90% and 70% coincidence with routine ELISA,IFA and CF respectively,while to 12 serum samples from healthy rabbits,it showed 100% coincidnce with ELISA,IFA and CF.All these suggested that Dot-ELISA may be a simple and convenient method for detecting serum IgG antibody against human and animal Lyme disease.

关 键 词：莱姆病 伯氏疏螺旋体 IGG 抗体ELISA 

分 类 号：R514.04[医药卫生—内科学] R446.62[医药卫生—临床医学]