牛蛙视网膜内γ-氨基丁酸免疫阳性反应的分布  

DISTRIBUTION OF GABA-LIKE IMMUNOREACTIVITY IN THE BULLFROG RETINA

作　　者：张军[1] 傅文 杨雄里[1] 

机构地区：[1]中国科学院上海生理研究所,上海200031

出　　处：《神经解剖学杂志》1999年第1期1-3,共3页Chinese Journal of Neuroanatomy

基　　金：中科院上海生理所开放实验室基金

摘　　要：用免疫组织化学ABC法．研究了GABA免疫阳性反应在牛蛙视网膜的分布。证明光感受器内段（主要是视锥细胞）呈棕褐色的GABA反应；在外核层未见GABA标记的胞体，但在靠近外网层处偶见GABA标记的终末；在内核层，大量无长突细胞呈GABA反应阳性，并可鉴别出胞体染色较深和淡的两个亚群，一些双极细胞和个别水平细胞的胞体及它们的突起呈较弱的GABA反应阳性，偶见双极细胞轴突终末以膨体紧密贴附在GABA标记的无长突细胞上。在节细胞层，一些神经节细胞和散在的移位无长突细胞呈GABA反应阳性。此外．外网层和内网层均呈GABA反应阳性。上述结果表明，GABA广泛分布于牛蛙视网膜的各层，提示它在视觉信号的传递过程中发挥着重要作用。Gamma-amino-butyric acid(GABA) is a major inhibitory transmitter in the vertebrate retina. The distribution of GABA-like immunoreactivity(GABA-IR) in bullfrog. Rana Calesbeiana, was studied with the immunohistochemical ABC method.The inner segment of photoreceptors (mainly cone). a few horizontal cells. some bipolar cells, numerous amacrine cells,including displaced amacrine cells, and a few ganglion cells showed GABA-IR. GABA-IR was also found intensely in the inner plexiform layer, weakly in the outer plexiform layer. This distribution pattern of GABA-IR in the bullfrog retina suggests that GABAmay play an important role in transmission and modulation of visual signals in both the outer and inner retina.

关 键 词：免疫组化 视网膜 牛蛙 Γ氨基丁酸 

分 类 号：Q959.530.5[生物学—动物学]