大鼠外周组织炎症、温热性痛觉过敏和脊髓强啡肽基因表达增强的相关关系──行为和mRNA的研究  

FUNCTIONAL RELATIONSHIPS BETWEEN PERIPHERAL INFLAMMATION,THERMAL HYPERALGESIA, AND ENHANCED DYNORPHIN GENE EXPRESSION IN SPINAL CORD OF RAT: BEHAVIORAL AND mRNA STUDIES

作　　者：张瑞新[1] 陈晋原[1] 乔健天[1] 

机构地区：[1]山西医科大学生理学教研室,太原030001

出　　处：《神经解剖学杂志》1999年第1期19-26,共8页Chinese Journal of Neuroanatomy

摘　　要：本文采用测量大鼠脚掌直径和后肢屈肌反射潜伏期以及Northernblot检测脊髓强啡肽mRNA的方法，研究了炎症反应-痛觉过敏-脊髓强啡肽mRNA表达的相关关系。发现将不同剂量的CFA注入大鼠一侧后脚掌后，该侧后脚掌即发生不同程度的炎症反应和痛觉过敏，二者之间呈正相关关系；同侧脊髓强啡肽mRNA表达增强，并与炎症反应和痛觉过敏均呈正相关关系。在注射不同剂量CFA诱发组织炎症反应、出现痛觉过敏和增加脊髓强啡肽mRNA表达时，似乎存在一个引发这些变化出现"跃变"的剂量。这些结果提示，持续的伤害性信息在脊髓水平的传递不是一个被动过程，而是一个包括背角环路中强啡肽能活动增强的中枢可塑性主动过程，从而导致产生痛觉过敏或／和痛觉记忆。防止这种对伤害性信息的传递起易化作用的改变，有利于防止外周组织损伤如手术损伤引起的痛觉过敏。The functional relationships among peripheral inflammation, thermal hyperalgesia, and dynorphin (DYN) gene expression in spinal cord of rats has been studied. The diameter of hindpaw and the withdrawal latency of hindpaw in response toradiant stimuli were measured as indicators of inflammation and hyperalgesia induced by unilateral complete Freund's adjuvant(CFA) injection into the hindpaw in rats. DYN mRNA was detected by Northern analysis. The study showed that CFA Injections dose dependently resulted in peripheral inflammation, hyperalgesia, and enhanced spinal DYN mRNA expression, showinga positive correlations among DYN mRNA expression, peripheral inflammation, and hyperalgesia. It also seems to have a threshold for inducing peripheral inflammation and activating the spinal DYN gene expression. These results indicate that the transmission of prolonged noxious inputs in the spinal cord is not a passive process but an aggressive one, which could induce a long-termplastic alterations of function and/or structure of central neurons, leading to the hyperalgesia and pain memory. It is believedthat pre-emptive blockade of these processes such as enhanced DYN mRNA expression in the spinal cord, would limit the alterations of central neurons and consequently alleviate the tissue damage (e. g. clinical surgery) induced hyperalgesia.

关 键 词：痛觉过敏 强啡肽 炎症反应 脊髓 

分 类 号：R338.3[医药卫生—人体生理学] R364.5[医药卫生—基础医学]