黑麦草叶内游离脯氨酸含量对于不同类型和强度的水分胁迫的生理生态响应(英文)  

PROLINE ACCUMULATION OF LEAVES OF PERENNIAL RYEGRASS ( LOLIUM PERENNE ) IN RESPONSE TO DIFFERENT TYPES AND LEVELS OF WATER STRESS:A PHYSIOLOGICAL ECOLOGY ASPECT

作　　者：高玉葆[1] 任安芝[1] 刘峰[1] 吴秀英[1] 

机构地区：[1]南开大学生物学系

出　　处：《植物生态学报》1999年第3期193-204,共12页Chinese Journal of Plant Ecology

摘　　要：实验植物黑麦草（Ｌｏｌｉｕｍｐｅｒｅｎｎｅ）种植于自行设计和制作的圆形土壤柱内，土壤含水量采用称重法加以控制，通过调节灌水次数和每次灌入水量模拟３种类型的水分胁迫，即周期性胁迫、持续稳定性胁迫和渐进性胁迫，并以充足灌水作为对照。３种胁迫又分别设有３个水平，即轻度、中度和重度。当土壤柱内植物建立稳定种群后开始胁迫处理，至生长季末共进行两个周期的胁迫实验。胁迫处理期间以固定间隔在草群内取最新完全展开的叶片测定其含水量和游离脯氨酸含量。实验结果表明，３种类型的水分胁迫均引起脯氨酸在植物叶内的积累且其数量随胁迫强度增加而变化，但这种效应是累积性的而不是即时性的。在任何一种处理的时间序列上，脯氨酸含量的变化与土壤含水量的变化不存在同步性，而与叶片含水量变化呈同步相反走势。通过与他人从生理生化角度所做的工作相比较，本文对上述研究结果的生理生态学意义进行了讨论。Perennial ryegrass was grown in soil column systems in which soil water content was monitored by regular weighing the column and was maintained within a predetermined range by controlling watering frequency and water amount.Three types of water stress were imposed after the simulated swards were well established,cyclic,sustained and progressive,each of which was further divided into mild,moderate and severe levels,and an adequately watered column was used as control.There were two periods of watering treatment in the growing season.Newly expanded leaf blades were sampled at regular intervals for the determination of water content and free proline concentration. There was a cumulative rather than an instant effect of water stress on proline accumulation in plant leaves,and different levels of stress caused different rates of accumulation.This effect became evident when the whole period of watering treatment was taken into account.Proline accumulation,on the time course,did not follow the pattern of soil water content variation but seemed to be negatively associated with the leaf water content.The physiological ecology significance of the results is discussed with particular reference to soil column use,age of swards and types and levels of wate stress.

关 键 词：脯氨酸 黑麦草 水分胁迫 生理生态 

分 类 号：S543.601[农业科学—作物学]