不同方法提取猪和牛卵母细胞和早期胚胎total RNA的比较  

Different Methods on Extracting Total RNA of Porcine and Bovine Oocytes and Early Embryos

作　　者：易康乐[1,2] 陈华海[3] 谢体三[3] 李纯锦[1] 李志才[2] 燕海峰[2] 石德顺[3] 周虚[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]湖南省畜牧兽医研究所,湖南长沙410131 [3]广西大学动物繁殖研究所,广西南宁530005

出　　处：《家畜生态学报》2010年第5期26-29,共4页Journal of Domestic Animal Ecology

基　　金：“863”计划项目(2008AA101003);国家科技支撑计划项目(2008BADB2B09-08)

摘　　要：研究以猪和牛GV期的卵母细胞和8细胞期胚胎作为研究对象,分别用QIAGENRNeasy Micro Kit、无根RNAPrep Micro kit和华舜快速RNA(微量)提取试剂盒提取其中的totalRNA,通过对total RNA的浓度和纯度的分析比较,找出较适宜的提取方法。结果显示,QIA-GEN RNeasy Micro Kit能更有效地从少量猪和牛卵母细胞和早期胚胎中提取高质量的totalRNA。Total RNA was extracted in germinal vesicle（GV）oocytes and 8-cell embrgos of pig and catte by using QIAGEN RNeasy Micro lxit,RNAPrep Micro Kit and Huashun rapid.RNA extracting reagent box（trace）.Through comparasion and analysis of the putity and denxity of total RNA,the better way of extracting was found.The results indicated that trace amount of total RNA with high quality can be extracted from small number of oocytes and embryos with QIAGEN RNeasy Micro Kit.

关 键 词：猪 牛 卵母细胞 早期胚胎 总RNA 

分 类 号：S811.5[农业科学—畜牧学]