调节性T细胞在老年阻塞性睡眠呼吸暂停低通气综合征中的作用  

The role of regulatory T cells in senile patients with obstructive sleep apnea hypopnea syndrome

作　　者：罗少华[1,2,3] 高兴林[1,2,3] 吴健[1,2,3] 林琦[1,2,3] 欧琼[1,2,3] 岑瑞金[1,2,3] 罗玲[1,2,3] 

机构地区：[1]广东省人民医院 [2]广东省医学科学院 [3]广东省老年医学研究所呼吸科,广州510080

出　　处：《广东医学》2010年第22期2907-2909,共3页Guangdong Medical Journal

基　　金：广东省科技计划项目(编号:2009B030801260);广东省医学科研基金资助项目(编号:B2009001)

摘　　要：目的了解CD4+ CD25+调节性T细胞(CD4+ CD25+ Treg)在老年阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea hypopnea syndrome,OSAHS)患者疾病发展过程中的作用。方法将老年(≥65岁)研究对象分为对照组、轻度OSAHS组、中度OSAHS组和重度OSAHS组(各11例),行多导睡眠图仪检查,以流式细胞仪测定外周血CD4+ CD25+ Treg占总T细胞的百分比(Treg%)作为CD4+ CD25+ Treg相对数,ELISA检测血清IL-6浓度。结果外周血Treg%在老年OSAHS重度组与中度组,皆较对照组及轻度组减少(P<0.05);血清IL-6浓度重度组较其余3组升高(P<0.05),中度组和轻度组皆较对照组升高(P<0.05);Treg%与呼吸暂停低通气指数(AHI)呈负相关(r=-0.615,P<0.001),与夜间最低血氧饱和度(LSaO2)呈正相关(r=0.604,P=0.031);血清IL-6浓度与AHI呈正相关(r=0.476,P=0.025),与LSaO2无相关关系(r=-0.365,P=0.183)。结论老年OSAHS患者外周血CD4+ CD25+调节性T细胞比例下降,抑制作用减弱,致炎因子IL-6浓度升高,可能参与了造成血管内皮损伤的炎症反应。Objective To explore the role of regulatory T cells in senile patients with OSAHS. Methods Senile subjects （≥65 years） were enrolled into control group,mild group,moderate group and severe groups （n=11） according to polysomnography （PSG）. Samples of peripheral venous blood were collected. ELISA and flow cytometry were applied to measure the levels of IL-6 in serum and percentage of CD4^＋CD25^＋Treg in T lymphocytes,respectively. Results The percentage of CD4^＋CD25^＋Treg in peripheral venous blood was significantly increased in patients with severe and moderate OSAHS compared with that in controls （P〈0.05）. IL-6 concentration was significantly elevated in severe OSAHS group,compared with those in all other groups （P〈0.05）,while significant increase of IL-6 was observed in moderate and mild OSAHS patients compared with that in control （P〈0.05）. Significant positive correlation and negative correlation were observed between percentage of CD4^＋CD25^＋Treg and AHI （r=-0.615,P〈0.001）,and LSaO2 （r=0.604,P=0.031）,respectively,while significant positive correlation observed between IL-6 concentration and AHI（r=0.476,P=0.025）. However,no significant correlation was found between IL-6 concentration and LSaO2 （r=-0.365,P=0.183）. Conclusion Percentage of CD4^＋CD25^＋Treg reduction and IL-6 concentration evaluation are observed in senile OSAHS patients,which may contribute to inflammation-induced vascular endothelium injury.

关 键 词：老年人 阻塞性睡眠呼吸暂停低通气综合征 呼吸暂停低通气指数 CD4+ CD25+调节性T细胞 白介素6 

分 类 号：R392.11[医药卫生—免疫学]