超临界CO_2萃取松花粉中的油脂及其GC分析  

SUPER-CRITICAL CO2 EXTRACTION OF OIL IN PINE POLLEN AND GC ANALYSIS

作　　者：杨万政[1] 路静[1] 张晓峰[2] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081 [2]承德医学院中药系

出　　处：《承德医学院学报》2010年第4期347-348,共2页Journal of Chengde Medical University

基　　金：北京高等学校化学实验教学示范中心建设项目(2008);中央民族大学本科专业建设项目(2008);中央民族大学教学改革项目(2008)

摘　　要：目的:研究油松花粉中脂肪酸的组成及含量。方法:采用超临界CO2流体萃取技术对松花粉进行萃取,采用酸甲酯化和碱甲酯化两种方式处理所得脂溶性成分并进行气相色谱分析。结果:酸甲酯化法处理得到8种脂肪酸,碱甲酯化法处理得到5种脂肪酸。结论:采用酸甲酯化方式处理较好。松花粉超临界CO2流体萃取物中主要成分为油酸、亚油酸、棕榈酸、硬脂酸和亚麻酸。Objective：To study the composition and content of fatty acids in pine pollen.Methods：Supercritical CO2 extraction technology was used to extract pine pollen. Acid esterification and alkaline esterification methods were used to treat, gas chromatography to analyze the obtained liposoluble constituents.Results：8 kinds of fatty acids were obtained using acid esterification method; while alkaline esterification method obtained 5 kinds.Conclusions：Compared with alkaline esterification method, acid esterification is fairly better. The main components of pine pollen are oleic acid, linoleic acid, palmitic acid, stearic acid and linolenic acid by supercritical CO2 extraction.

关 键 词：松花粉 超临界CO2 萃取 酸甲酯化 碱甲酯化 气相 

分 类 号：R917[医药卫生—药物分析学]