穿山龙总皂苷对CIA大鼠滑膜血管新生的作用  

EFFECTS OF TOTAL SAPONIN OF RHIZOMA DIOSCREAE NIPPONICAE ON ANGIOGENESIS IN SYNOVIAL MEMBRANE OF CIA RATS

作　　者：董文娟[1] 梁秀军[1] 翟泽玲[1] 宋鸿儒[1] 

机构地区：[1]承德医学院,河北承德067000

出　　处：《承德医学院学报》2010年第4期360-362,共3页Journal of Chengde Medical University

基　　金：国家自然基金项目(30873420);河北省自然基金重点项目(C2007000916)

摘　　要：目的:探讨穿山龙总皂苷对胶原诱导性关节炎(collagen-induced arthritis,CIA)大鼠滑膜血管新生及血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的作用。方法:42只Wistar大鼠随机分为7组:正常对照组(A组)、CIA模型组(B组)、雷公藤组(C组)、穿山龙总皂苷高剂量组(D组)、穿山龙总皂苷中剂量组(E组)、穿山龙总皂苷低剂量组(F组)和薯蓣皂苷元组(G组),每组6只大鼠。B-G组大鼠均建立CIA大鼠模型,待模型成功建立后,C-G组大鼠分别给予相应的药物灌胃,连续21d。采用免疫组织化学染色法检测关节滑膜的微血管密度(MVD)和VEGF的表达。结果:CIA模型组大鼠滑膜MVD和VEGF的表达显著高于正常对照组(P<0.01;)C-G组大鼠滑膜MVD和VEGF的表达明显低于模型组(P<0.01),且各治疗组间比较无显著差异(P>0.05)。结论:穿山龙总皂苷可能通过降低关节滑膜VEGF的表达发挥抑制滑膜血管新生的作用。Objective：To investigate effects of total saponin of Rhizoma Dioscreae Nipponicae （RDN） on angiogenesis and expression of vascular endothelial growth factor （VEGF） in synovial membrane of collageninduccd arthritis （C1A） rats.Methods：42 Wistar rats were randomly divided into 7 groups （n=6）： normal control group （A）, CIA model group （B）, Tripterygium group （C）, RDN high dose group （D）, RDN middle dose group （E）, RDN low dose group （F） and Diosgenin group （G）. Rats in B-G group were established CIA rat model; then the rats in C-G group were lavaged with corresponding medicine for 21 days. Immunohistochemical staining was used to detect MVD and VEGF expression in synovial membrane.Results：MVD and VEGF expression in synovial membrane of CIA rats were obviously higher than normal control rats （P〈 0.01）; MVD and VEGF expression in synovial membrane of rats in each treatment group were obviously lower than CIA model rats （P〈 0.01）; moreover, there had no obvious differences between each treatment group （-P〉 0.05）. Conclnsions：RDN can inhibit angiogenesis in synovial membrane by down regulating VEGF expression.

关 键 词：CIA 穿LIJ龙总皂苷 血管新生 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]