高效液相色谱法测定化妆品中的泛酸及D-泛醇  

Simultaneous determination of pantothenic acid and D-panthenol in cosmetics by high performance liquid chromatography

作　　者：毛希琴 胡侠 潘炜 

机构地区：[1]大连市产品质量监督检验所,辽宁大连116023

出　　处：《色谱》2010年第11期1061-1066,共6页Chinese Journal of Chromatography

基　　金：国家标准计划项目(No.20081995-T-607)

摘　　要：建立了对不同基质化妆品(膏霜、乳液、水剂化妆品、油剂化妆品、蜡基化妆品、指甲油等)中泛酸(维生素B5)及D-泛醇(维生素原B5)的富集方法及同时测定的高效液相色谱(HPLC)法。利用水和与水不互溶的有机溶剂组成的双液相体系,首先将泛酸及D-泛醇与化妆品中油溶性成分及表面活性剂等基质成分初步分离,然后用亚铁氰化钾-乙酸锌共沉淀剂去除提取液中的水溶性成分,继而在酸性条件下将泛酸和D-泛醇富集于C18固相萃取填料上,脱除其他水溶性干扰物后,用40%甲醇水溶液洗脱,用HPLC分离,紫外检测器检测,外标法定量。该方法在泛酸和D-泛醇的含量为0.1～10μg/g范围内有很好的线性,线性相关系数分别为0.9989和0.9996。不同化妆品基质中目标成分的方法回收率均在90%以上。对泛酸及D-泛醇的检出限均为30μg/g,定量限均为100μg/g。实验表明该方法可用于化妆品中泛酸及D-泛醇的同时测定,结果准确可靠。A high performance liquid chromatographic method（HPLC）and sample pretreatment method were developed for the simultaneous determination of pantothenic acid（vitamin B5）and Dpanthenol（provitamin B5）in cosmetics with different matrices（including of creams,lotions,aqueous cosmetics,oily cosmetics,wax-based cosmetics,nail polish etc）.A liquid-liquid extraction system composed of water and water-immiscible solvent was used to preliminarily separate the target components from other oil-soluble components and surfactants in cosmetics,then macromolecular water-soluble matrices in cosmetics were removed by coprecipitation with potassium ferrocyanide-zinc acetate precipitating agent,and then under acid condition,pantothenic acid and D-panthenol were enriched on a C18 solid-phase extraction sorbent.After the removal of other water-soluble impurities,target components were eluted by 40% methanol and then separated and quantitatively analyzed by high performance liquid chromatography with external standard method.Good linear relationship was achieved in the concentration range of 0.1-10μg/g for pantothenic acid and D-panthenol.The linear correlation coefficients were separately 0.998 9 and 0.999 6.The average recoveries of the target components in cosmetics were more than 90%.Limit of detection of the method was 30μg/g and the limit of quantification was 100μg/g.This method can be used to simultaneously determine pantothenic acid and D-panthenol in cosmetics.The results are accurate and reliable.

关 键 词：高效液相色谱法 泛酸 D-泛醇 化妆品 

分 类 号：O658[理学—分析化学]