毛细管电泳-安培检测法测定3种拟肾上腺素药物  

Determination of three adrenergic drugs using capillary electrophoresis with amperometric detection

作　　者：黄颖[1] 张晓丽[1] 占春荣[1] 陈国南[2] 

机构地区：[1]福建师范大学化学与材料学院,福建福州350108 [2]福州大学食品安全分析与检测教育部重点实验室,福建福州350108

出　　处：《色谱》2010年第11期1084-1088,共5页Chinese Journal of Chromatography

基　　金：国家自然科学基金项目(No.20877019);福建省属高校科研专项计划项目(JK2010008)

摘　　要：建立了毛细管电泳-安培检测法测定盐酸去氧肾上腺素(phenylephrine hydrochloride,PHE)、重酒石酸间羟胺(metaraminol bitartrate,MR)和盐酸异丙肾上腺素(isoprenaline hydrochloride,IP)3种拟肾上腺素药物的方法。检测电位为0.950V(Ag/AgCl为参比电极),硼酸盐浓度为50mmol/L(pH10.00),分离电压为18kV,进样时间为10s。在最佳实验条件下,3种物质在18min内达到基线分离,在2～100μmol/L浓度范围内峰面积与浓度呈良好的线性关系,线性相关系数不小于0.9991。盐酸去氧肾上腺素、重酒石酸间羟胺和盐酸异丙肾上腺素的检出限分别为0.8、0.8和1.0μmol/L。将所建立的方法应用于针剂样品的分析,结果令人满意。A method for the determination of three adrenergic drugs,including phenylephrine hydrochloride（PHE）,metaraminol bitartrate（MR）and isoprenaline hydrochloride（IP）,was developed using capillary electrophoresis with amperometric detection.The detection potential of working electrode was 0.950 V versus the reference electrode of Ag/AgCl.At the applied voltage of 18 kV,the three analytes were completely separated within 18 min in 50 mmol/L borate buffer（pH10.00）with the injection time of 10 s.Good linear relationships were obtained for all the three analytes in the range of 2-100μmol/L.The detection limits for PHE,MR and IP were 0.8,0.8 and 1.0μmol/L,respectively.The proposed method was applied to the analysis of some injection drugs,and the results were satisfactory.

关 键 词：毛细管电泳法 安培检测 拟肾上腺素药物 

分 类 号：O658[理学—分析化学]