猪繁殖与呼吸综合征病毒变异株分离鉴定及遗传变异分析  

Isolation,identification and genetic vatiation analysis of the mutation strain of porcine reproductive and respiratory syndrome virus

作　　者：沈宪文[1,2] 李月红[3] 王晓莉[1] 宋德武[1] 宣华[4] 王贵平[4] 母连志[1] 丁壮[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林农业科技学院,吉林吉林132101 [3]吉林农业大学动物科技学院,吉林长春130118 [4]广东省农业科学院兽医科学研究所,广东广州510000

出　　处：《中国兽医学报》2010年第11期1409-1412,共4页Chinese Journal of Veterinary Science

基　　金：广东省农业科技重点项目(2008A020100020)

摘　　要：从临床＂高热综合征＂病例分离到1株猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syn-drome,PRRSV）,经分离培养、血清学鉴定和分子病毒学鉴定,确定为美洲型猪繁殖与呼吸综合征病毒,并命名为DZ0908。设计了针对病毒Nsp2基因和GP5基因的引物,扩增出目的基因。通过序列比对进行遗传变异分析,该分离株Nsp2基因序列中第483位和第535~563位氨基酸存在缺失,GP5相对保守,该毒株属于PRRSV变异株,为该病毒的进一步研究奠定了基础。One strain of porcine reproductive and respiratory syndrome virus（PRRSV） was isolated from a pig with the clinical ＂high fever syndrome＂,named DZ0908,and identified as a strain of American type PRRSV according to the isolating culture,serology and molecular virology.Two pairs of primers were designed for the amplification of nonstructural protein 2（Nsp2） gene and GP5 gene.The sequence analysis revealed that the isolated virus was a mutation strain as the Nsp2 gene existed amino acid deletion in the position of 483 and 535-563 and the GP5 gene was conservative relatively.The data may form a foundation for the future investigation.

关 键 词：猪繁殖与呼吸综合征病毒 分离鉴定 遗传变异分析 

分 类 号：S852.65[农业科学—基础兽医学]