BDNF和NGF对牛颗粒细胞体外生长的影响  

Effects of NGF and BDNF on proliferation of bovine granulosa cells

作　　者：易康乐[1,2] 陈华海[3] 李纯锦[1] 孙艳玲[1] 陈露[1] 石德顺[3] 周虚[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]湖南省畜牧兽医研究所,湖南长沙410131 [3]广西大学动物繁殖研究所,广西南宁530005

出　　处：《中国兽医学报》2010年第11期1544-1546,共3页Chinese Journal of Veterinary Science

基　　金：国家"863"计划资助项目(2008AA101003);国家科技支撑计划资助项目(2008BADB2B09-08)

摘　　要：在DMEM培养液中分别添加不同质量浓度的脑源性神经生长因子(Brain-derived neurotrophic factor,BD-NF)和神经生长因子(Nerve growth factor,NGF),观察其对牛颗粒细胞体外生长的影响。结果显示:在DMEM中添加20μg/L的BDNF,可促进牛颗粒细胞的体外生长;在DMEM中添加5μg/L的NGF,可促进牛颗粒细胞的体外生长。结果表明,在DMEM培养液中添加一定质量浓度的NGF和BDNF可促进牛颗粒细胞的体外生长。Effects of BDNF and NGF concentrations on proliferation of bovine granulosa cells were studied.Supplementation of DMEM cultured media with BDNF at the concentration of 20 μg/L or NGF at the concentration of 5 μg/L caused a significant increase in number of granulosa cells after cultrued for 48 h,indicating that the NGF and BDNF can promote in vitro proliferation of bovine granulosa cells.

关 键 词：BDNF NGF 牛 颗粒细胞 

分 类 号：S814.8[农业科学—畜牧学]