草原红牛肌生成抑制素基因内含子1多态性及部分屠宰性状的相关性分析  

Polymorphism of myostatin intron 1 gene and its associations with several carcass traits in red steppe

作　　者：张胜[1] 张永宏[1] 高妍[2] 张犁苹[1] 刘同欣[1] 秦莹[1] 马倩[1] 张嘉保[2] 赵志辉[1] 张国梁[3] 邢沈阳[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062 [3]吉林省农业科学院畜牧分院,吉林公主岭136100

出　　处：《中国兽医学报》2010年第11期1557-1560,共4页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2007BAD55B01);转基因生物新品种培育科技重大专项资助项目(2008ZX08007-001)

摘　　要：以草原红牛为研究对象,采用PCR-SSCP方法检测肌生成抑制素(MSTN)基因内含子1基因的多态性。结果表明:在内含子1发现多态性位点,有AA、AB和BB 3种基因型;测序结果显示在1 452 bp处存在C→T的碱基突变;该位点的多态性对草原红牛的净肉率有显著影响(P<0.05),BB型和AB型显著高于AA型(P<0.05),BB型和AB型之间差异不显著(P>0.05);其他屠宰性状在不同基因型间差异不显著(P>0.05)。由此可以初步推断MSTN基因可能是影响草原红牛肉质性状的一个主效基因或是一个影响肉质性状的QTL连锁的基因。This study was designed to investigate the single nucleotide polymorphism（SNP） of myostatin（MSTN） gene in red steppe and to analyze by PCR-SSCP,at the same time the correlation between the polymorphisms and several carcass traits.The results of cloning and sequencing indicated that polymorphism of intron 1 dued to base mutation of C→T in position 1 452 bp,and there were three genotypes（AA,AB and BB）.The correlation analysis revealed that polymorphisms had a significant effect on net meat percentage,the BB and AB genotype had higher net meat percentage than the AA genotype in red steppe（P0.05）,but there were no significant differences between BB genotype and AB genotype（P0.05）.Other carcass traits of red steppe was not significant in different genotypes（P0.05）.It could be preliminarily concluded that MSTN gene was probably a major gene or a QTL linked gene which associated with meat quality traits in red steppe.

关 键 词：草原红牛 肌生成抑制素基因 PCR-SSCP 屠体性状 

分 类 号：S814[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]