骨髓间充质干细胞移植对大鼠急性心肌梗死后心功能及bcl-2和caspase-3 mRNA表达水平的影响  

Effects of MSCs transplantation on cardiac function and expression of bcl-2 and caspase-3 mRNA in the post-AMI rats

作　　者：谭晓[1] 王迪斌[1] 卫慧[1] 朱嵘[1] 黄晓丹[1] 杨志健[1] 

机构地区：[1]南京医科大学第二附属医院心内科,江苏南京210011

出　　处：《实用临床医药杂志》2010年第11期1-4,共4页Journal of Clinical Medicine in Practice

摘　　要：目的观察急性心肌梗死大鼠应用骨髓间充质干细胞(MSCs)移植前后心功能及凋亡相关蛋白bcl-2和caspase-3mRNA的变化,以期了解骨髓间充质干细胞移植治疗心梗的机制。方法 40只SD大鼠,雌雄各半,随机分为对照组、假手术组、心梗组、移植组,每组各10只。心梗组和移植组大鼠分别结扎前降支造成心梗模型。取大鼠骨髓,体外分离扩增培养MSCs。术前4组大鼠进行超声心动图检测心功能,结扎后移植组立即予MSCs多点注射到大鼠心肌梗死区周边,而梗死组和假手术组注射同剂量培养液,对照组不注射。饲养8周后,分别予超声心动图心功能检测。取梗死周边区心肌行RT-PCR检测bcl-2和caspase-3 mRNA的表达。结果术前4组大鼠心功能指数无显著差异(P>0.05);而MSCs移植后,梗死组大鼠心肌LVESD、LVEDD较其它3组明显增高,差异具有显著性(P<0.05);而移植组又较对照组和假手术组明显增高(P<0.05);而对照组和假手术组在上述指标中无显著差异(P>0.05);梗死组大鼠EF较其它3组明显降低(P<0.05);而移植组又较对照组、假手术组明显降低(P<0.05);而对照组与假手术组之间无显著差异(P>0.05)。bcl-2mRNA在对照组和假手术组表达较少,而在梗死组表达增加,差异有显著性(P<0.05);在移植组表达更多,与梗死组相比有显著性(P<0.05);caspase-3在对照组和假手术组表达较少,在梗死组表达增加,有显著差异(P<0.05);在移植组表达又较梗死组表达减少,有显著差异(P<0.05);但与对照组和假手术组相比,表达增加(P<0.05)。结论急性心肌梗死移植MSCs后,心功能得到改善,心肌凋亡得到抑制。Objective To study the mechanism of MSCs transplantation treating myocardial infarction by observing the change of cardiac function and apoptosis related protein bcl-2 and caspase -3mRNA before and after MSCs transplantation. Methods A total of 40 SD rats （female/male = 1： 1） were randomly divided into four groups which were Control Group, Sham-operation Group, MI Group and Transplant Group. In MI Group and Transplant Group, descending anterior branches were ligated to make MI models. Derived bone marrow from rats, then isolate and expand to culture MSCs. Before ligature, the cardiac function of 4 groups rats were tested by ultrasonic cardiogram. After ligature, immediately give multi-dot injection of MSCs around myocardial infarction area only for the rats in Transplant Group, while injecting the same dosage of nutrient solution in MI Group and Sham-operation Group, and no injection for Control Group. Then after 8 weeks, test the cardiac function of rats separately and derive myocardium around infraction area to test the expressions of bcl - 2 and caspase - 3 mRNA by RT - PCR. Results Before ligature , there was no significant difference in cardiac function index among 4 groups （P 〉0.05）, while after transplant of MSCs, LVESD and LVEDD of MI Group were obviously higher than those of the other 3 groups （P〈0. 05）, and Transplant Group was significantly higher than Control Group and Sham-operation Group （P 〈0.05）. However between Control Group and Sham-operation Group, there was no significant difference （P 〉0.05）. EF of MI Group was significantly lower than that of the other 3 groups （ P 〈 0.05）, Transplant Group was obviously lower than Control Group and Sham-operation Group （ P 〈 0.05）, and there was no significant difference between Control Group and Sham-operation Group（P 〉0.05）. Expression of bcl-2mRNA was less in Control Group and Sham-operation Group, but increasing in MI Group with significant difference （P 〈 0.05）, while expression of bcl-2mRNA was muc

关 键 词：急性心肌梗死 骨髓间充质干细胞 心功能 

分 类 号：R542.2[医药卫生—心血管疾病]