大鼠胰岛细胞经门静脉肝内移植模型的建立与应用  

lntrahepatic islet transplantation through the portal vein in diabetic rats

作　　者：杨顺良[1] 吴志贤[1] 叶永峰[1] 蔡锦全[1] 王庆华[1] 黄梁浒[1] 林国章[1] 周浩[1] 谭建明[1] 

机构地区：[1]南京军区福州总医院泌尿外科,福建医科大学福总临床教学医院,全军器官移植中心,福建省移植免疫重点实验室,福州350025

出　　处：《中华细胞与干细胞移植（电子版）》2010年第1期4-7,共4页

基　　金：福建省科技计划重点项目（2005Y017）,国家自然科学基金（30571759）：全军“十一五”计划课题（06G037）

摘　　要：目的建立一种经门静脉胰岛细胞肝内移植治疗远交群（SD）大鼠1型糖尿病的动物模型。方法链尿佐菌素诱导SD大鼠建立1型糖尿病模型20只,分为两组：实验组15只,分别经门静脉输注SD大鼠胰岛1000IEQ（1.5ml）;对照组5只,分别经门静脉输注无血清1640液1.5ml。移植术后未服用免疫抑制剂,观察出血量、移植术后血糖、尿量和体重等指标。结果所有大鼠均成活,门静脉穿刺一次成功率90﹪,出血量〈0.5ml。胰岛移植大鼠血糖降为正常的时间为1～6d,平均（3.7±1.7）d。移植胰岛存活时间为2～15d,平均（8.4±4.1）d。移植术后2周内移植组大鼠空腹血清胰岛素明显高于对照组（P〈0.01）。肝脏病理检查显示胰岛细胞在肝窦内存活并能发挥功能。结论大鼠胰岛经门静脉肝内移植是胰岛移植基础研究的理想模型。Objectives To investigate the efficiency of intrahepatic islet transplantation through portal vein in diabetic rats. Methods Type 1 diabetes mellitus was induced with streptozotocin in 20 rats, 15 of which were infused with islets （1000 IEQ, 1.5 ml） through the portal vein respectively as experimental groups and 5 were infused with serum-free 1640 medium （1.5 ml ） respectively as control. No immunosuppressive drug was administered posttransplant. Bleeding volume, blood glucose, urine volume and weight were monitored. Results All rats survived. Success rate of portal puncture was 90 % with bleeding volume less than 0.5 ml. Duration of normal blood glucose posttransplant in experimental group was 1 to 6 days （3.7 ± 1.7 days）. The islet graft survival time was 2 to 15 days （8.4 ± 4.1days）. Serum insulin levels in experimental group were significantly higher compared with control within 2 weeks posttransplant. Islet graft was viable and functioning in hepatic sinusoids. Conclusions Intrahepatic islet transplantation through the portal vein in rats is feasible.

关 键 词：糖尿病 胰岛 移植 门静脉 大鼠 

分 类 号：R699.2[医药卫生—泌尿科学]