NGAL预测CO_2气腹对大鼠早期肾脏的影响  

NGAL predicts early kidney injury induced by CO_2 pneumoperitoneum in rats

作　　者：姜华琴[1] 尹忠诚[2] 

机构地区：[1]徐州医学院,221000 [2]徐州医学院附属医院肾脏病科,221000

出　　处：《江苏医药》2010年第20期2422-2424,F0003,共4页Jiangsu Medical Journal

基　　金：江苏省卫生厅医学科技发展基金(H200833)

摘　　要：目的探讨中性粒细胞明胶酶相关脂笼蛋白(NGAL)在气腹继发早期急性肾脏损伤(AKI)中的预测作用。方法雄性SD大鼠50只随机均分5组,按不同气腹压值和持续时间建立CO2气腹模型。全自动生化分析仪检测气腹前后尿素氮(BUN)、血肌酐(SCr);ELISA法检测血清和尿中NGAL含量,光镜下肾组织病理检查及损伤半定量评分。结果 SCr、BUN各组气腹前后及组间比较差异无统计学意义(P>0.05);血清和尿NGAL各组气腹前后及与对照组比较均有明显升高(P<0.05);各气腹组肾损伤评分明显高于对照组(P<0.05),且与气腹后即刻血清和尿NGAL显著相关(P<0.01)。结论检测血清和尿中NGAL含量能早期预测大鼠CO2气腹后AKI。Objective To observe the value of expression of neutrophil gelatinase-associated-lipocalin（NGAL）in predicting early acute kidney injury（AKI） induced by CO2 pneumoperitoneum.Methods Fifty male SD rats were divided randomly into five groups with 10 rats each.Besids sham operation control group,the models of CO2 pneumoperitoneum in four experimental groups were established with different pressures and durations of inflation.NGAL expression was detected by ELISA and renal function was estimated by quantitative analysis.The kidney injury was histologically evaluated.Results Serum creatine（SCr）and blood urine nitrogen（BUN）were not significantly differenct before and after pneumoperitoneum or among five groups（P〉0.05）.Compared to control group,NGAL expressions increased significantly after pneumoperitoneum（P〈0.05）.So did the scores of histological evaluations.Serum and urine NGAL expression was closely correlated to the degree of tubulointerstitial lesions（P〈0.01）.Conclusion Detection of NGAL in serum and urine may predict early AKI induced by CO2 pneumoperitoneum in rats.

关 键 词：中性粒细胞明胶酶相关脂笼蛋白 CO2气腹 急性肾损伤 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]