黄芪总苷对大鼠神经元缺氧/复氧损伤的影响  

Protective Effect of Astragalosides on Anoxia/Reoxygenation Injury of Hippocampal Neuron

作　　者：尹艳艳[1] 朱芬芳[1] 吴国翠[1] 李维祖[1] 公惠玲[1] 李卫平[1] 

机构地区：[1]安徽医科大学药理学教研室,合肥230032

出　　处：《中国中西医结合杂志》2010年第11期1173-1177,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：安徽省教育厅自然科学基金(No.KJ2008B301);安徽省高等学校青年教师科研资助项目(No.2008jq1059zd);安徽省人才开发基金资助项目(No.2007Z0301);安徽省自然科学基金项目(No.00144414)

摘　　要：目的探讨黄芪总苷(astragalosides,AST)对大鼠神经元缺氧/复氧损伤的影响。方法建立原代培养的大鼠海马神经元的缺氧/复氧模型。采用四唑蓝(MTT)法和乳酸脱氢酶(LDH)释放法检测细胞活力;检测细胞上清液中丙二醛(MDA)和一氧化氮(NO)含量、超氧化物歧化酶(SOD)活性;Hoechst染色和AnnexinV/PI双染流式细胞术检测细胞凋亡;采用激光共聚焦显微镜和Ca2+荧光探针Fluo-3/AM观察细胞内游离钙[Ca2+]i。结果黄芪总苷可以提高缺氧/复氧后神经元的活力,降低细胞上清液中MDA和NO含量,提高SOD活性,降低细胞凋亡率,减轻神经元内的钙超载。结论黄芪总苷对缺氧/复氧损伤的神经元具有保护作用,其机制可能与抗氧化和减轻钙超载有关。Objective To study the effect of astragalosides（AST） on the anoxia/reoxygenation（A/R） injured neuron in rat.Methods Primary cultured rat’s hippocampal neurons were made into A/R model cells.The cell viability was detected by MTT assay and lactate dehydrogenase releasing methods;the activity of superoxide dismutase（SOD）,and contents of malondialdehyde（MDA） and nitride oxide（NO） in culture supernate were detected;the apoptosis rate of hippocampal neurons after A/R was measured by flow cytometry with double-staining of hoechst33258 and AnnexinVPI;and intracellular calcium ion [Ca2＋]i was observed with a cofocal laser-scanning microscope and determined by fluorescent probe Fluo-3/AM.Results AST enhanced the cell viability of neurons after A/R injury,increased SOD activity and decreased the MDA and NO contents in supernate,reduced the A/R-induced apoptosis and decreased the calcium overload in neurons.Conclusion AST has the protective effects on A/R injured neurons,the mechanism is possibly related with its anti-oxidation and calcium overload reducing actions.

关 键 词：黄芪总苷 细胞培养 缺氧/复氧 钙 

分 类 号：R285.5[医药卫生—中药学]