单侧隐睾大鼠对侧睾丸组织中SCF/c-kit基因表达变化及意义  

Pathologic changes and expressions of SCF and c-kit in contralateral testes in rat model of unilateral cryptorchidism

作　　者：徐明[1] 郑新民[2] 李世文[1] 丁协刚[1] 周克文[1] 汪聪[1] 

机构地区：[1]武汉大学中南医院胸外科,431071 [2]武汉大学中南医院泌尿外科,431071

出　　处：《中华小儿外科杂志》2010年第11期859-862,共4页Chinese Journal of Pediatric Surgery

摘　　要：目的 研究单侧隐睾大鼠对侧睾丸病理变化及SCF/c-kit基因表达,探讨单侧隐睾致对侧睾丸损害的机制.方法 30只SD大鼠随机分为对照组和实验组,实验组复制单侧（左侧）腹腔隐睾模型.3个月后分别取两组右侧睾丸组织进行real-time RT-PCR、Western blot及免疫组化检测干细胞生长因子（SCF）和其受体c-kit基因及其蛋白表达变化,TUNEL法检测细胞凋亡.结果 所有动物均存活,与对照组相比实验组对侧睾丸明显缩小,光镜下观察其曲细精管发生退化,生精上皮变薄,管腔较空,生殖细胞明显减少,细胞凋亡增加.两组凋亡指数分别为14.4±0.63和4.45±0.37,差异有统计学意义（P＜0.05）.荧光实时定量PT-PCR检测SCF、c-kit基因mRNA含量,实验组对侧睾丸明显降低,两组相比差异有统计学意义（P＜0.05）.Western blot检测SCF及c-kit蛋白表达含量,实验组对侧睾丸同样明显降低,两组相比差异有统计学意义（P＜0.05）.免疫组化染色显示各级生精细胞膜均有c-kit表达,SCF主要表达于支持细胞膜表面,但实验组两者的表达均较对照组减弱.相关性检验SCF与AI相关系数r=-0.941,P＜0.01;c-kit与AI相关系数r=-0.908,P＜0.01;SCF与c-kit相关系数r=0.956,P＜0.01,均有统计学意义.结论 单侧隐睾可致对侧睾丸SCF/c-kit基因表达减弱,生精细胞凋亡增加引起不育.Objective To investigate the pathologic changes and expressions of SCF and c-kit in the contralateral testes in rat model of unilateral cryptorchidism. Methods Thirty male SD rats were maintained under controlled temperature and constant photoperiodic conditions with access to food and water. The rats were randomly assaigned to the control group and the experimental unilateral cryptorchidism group. The left testis of the rats in the unilateral cryptorchidism group was placed into the abdominal cavity. The control group rats were subjected to sham surgery. Three months later, the rats were sacrificed and their right testes were harvested. The pathological changes were observed under microscope. The mRNA and protein expression of SCF and c-kit were also investigated using quantitative Real-time RT-PCR, Western blotting and immunohistochemical staining. Apoptotic germ cells were detected by TUNEL staining. Results All rats survived to the endpoints. The right testes of rats of the unilateral cryptorichid group were smaller than those ot the control group. Normal testes of rats of the unilateral cryptorichid group were smaller than those of the control group. Normal testes of the control group rats manifested active spermatogenesis and orderly arrangement of germ ceils. However, disordered and sloughed germinal cells with less distinct seminiferous tubule borders were observed in the eontralateral testes of rats with experimental unilateral cryptorichid under the microscope. Cmpared with the control testes, the mRNA and protein expression of SCF and c kit of the contralateral testes of rats with experimental unilateral cryptorichid was decreased （P〈0. 05）. The apoptotic germ cells were increased （19. 7 ± 3.83 vs 5.4 ± 1.02, P〈0. 05）. The SCF/c-kit expression was positively correlated with the germ cell apoptosis （P〈0. 01 ）. Conclusions The decreased expres- sion of SCF and c-kit and increased germ cell apoptosis are found in the contralateral testes, which may contribute to the infertility in the

关 键 词：单侧隐睾 对侧睾丸 干细胞因子 受体 

分 类 号：R686[医药卫生—骨科学]