慢性HBV感染者CD8^+ T细胞免疫反应的研究  

CD8^+ T cell immune response in peripheral blood of patients with chronic HBV infection

作　　者：何长伦[1] 王寿明[1] 张锦海[2] 吕恒[2] 郑纪山[2] 

机构地区：[1]南京解放军第八一医院全军肝病中心,210002 [2]南京军区联勤部军事医学研究所

出　　处：《肝脏》2010年第5期332-334,共3页Chinese Hepatology

基　　金：国家自然科学基金资助项目(30671929)

摘　　要：目的观察不同慢性HBV感染者外周血CD8+ T细胞的细胞内毒性颗粒和细胞因子的表达水平。方法用rHBcAg作为刺激剂,采用免疫荧光染色结合流式细胞术分析慢性乙型肝炎中度、重度患者以及无症状HBV携带者外周血CD8+ T细胞的IFN-γ/IL-4和穿孔素/颗粒酶B表达水平。结果慢性乙型肝炎重度患者CD8+T细胞的穿孔素及颗粒酶B百分率显著高于正常对照(P<0.01)和HBV携带者(P<0.05,P<0.01),中度患者显著高于正常对照(P<0.05)。2组慢性乙型肝炎IFN-γ+/CD8+ T细胞(Tc1)百分率均高于正常对照和HBV携带者(P<0.05)。HBV携带者CD8+T细胞的穿孔素和颗粒酶B表达以及Tc1百分率与健康对照差异无统计学意义。IL-4+/CD8+ T细(Tc2)表达各组间差异无统计学意义(P>0.05)。结论 CD8+ T细胞中穿孔素/颗粒酶B表达率的增加以及Tc1细胞增加与慢性HBV感染的肝损伤有关。Objective To investigate the levels of intracellular cytokines and cytotoxic granules in CD8^＋T cell of patients with chronic HBV infection. Methods After stimulation with rHBcAg,the expressions of IFN-γ/ IL-4 and perforin（PFN） /granzyme B（Gzm B） in peripheral blood CD8^＋ T cell were analyzed by flow cytometry. Results The percentages of PFN/Gzm B in CD8^＋ T cells were significantly higher in patients with chronic hepatitis moderate（CHM）and chronic hepatitis severe（CHS） than those in normal control（NC） （P〈0.01,P〈0.05）. However,there was no significant difference in PFN/Gzm B in CD8^＋ T cells between HBV carriers and NC. The frequency of IFN-γ＋ /CD8^＋ T cells（Tc1） were significantly higher in CHS and CHM than those in NC（P〈0.05）. There was no significant difference in frequency of IL-4^＋/CD8^＋ T（Tc2） among different groups （P〈0.05）. Conclusion The increases in percentages of PFN/Gzm B in CD8^＋ T cells and Tc1 cells were related to liver injury during chronic HBV infection.

关 键 词：乙型肝炎 CD8 T细胞 细胞因子 颗粒酶B 穿孔素 

分 类 号：R512.62[医药卫生—内科学]