低频经颅磁刺激对颞叶癫海马超微结构的影响  

Effects of Low Frequency Repetitive Transcranial Magnetic Stimulation on the Hippocampal Ultrastructure in Rat Model of Temporal Lobe Epilepsy

作　　者：宋毅军[1] 田小冰[1] 田心[2] 

机构地区：[1]天津医科大学总医院神经内科,300052 [2]天津医科大学生物医学工程系

出　　处：《天津医药》2010年第11期977-979,1026,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30770545);天津市自然科学基金资助项目(项目编号:10JCYBJC13800);天津医科大学新世纪人才支持计划资助项目

摘　　要：目的:研究低频经颅磁刺激(rTMS)对颞叶癫大鼠海马超微结构的影响。方法:用清洁级Wistar大鼠制备氯化锂-匹罗卡品诱导的颞叶癫大鼠模型,随机分为对照组和rTMS组,每组5只,对rTMS组大鼠进行rTMS治疗。利用电子显微镜从神经元、神经纤维、突触、胶质细胞和毛细血管5个方面观察颞叶癫对海马超微结构的病理损伤和rTMS对颞叶癫海马区病理损伤的作用。结果:颞叶癫对海马区超微结构具有明显病理损伤,包括神经元出现凋亡和坏死,核浓缩,染色质聚集,线粒体嵴断裂,神经细胞和胶质细胞突起明显水肿,神经纤维局部髓鞘降解,轴浆内微管、微丝排列紊乱,血管内皮细胞肿胀,胶质细胞损伤。rTMS后海马超微结构明显改善,包括脑组织水肿减轻,凋亡和损伤细胞减少,突触和神经毡病变改善和血管水肿减轻。结论:rTMS可改善颞叶癫对海马超微结构的病理损伤,具有神经保护作用。Objective：To study the effects of low frequency repetitive transcranial magnetic stimulation （rTMS） on the hippocampal ultrastructure in rat model of temporal lobe epilepsy（TLE）. Methods：Ten Wistar rats were randomly divided into two groups, TLE group and rTMS group. The rat model of TLE was induced by lithium-pilocarpine. The ultrastructural changes in hippocampus were observed with electron microscope in rats of both groups. Results：There was significant pathological damage in the hippocampal ultrastructure, including neuronal apoptosis and necrosis, nuclear condensation, chromatin aggregation, mitochondrial cristae fracture, edema of nerve cells and glial cells, degradation of local medullary sheath of nerve fibers, disarrangement of microtubule and microfilaments of axoplasm, vascular endothelial cell swelling and glial cell damage in rats of TLE group. The pathological damages were significantly improved after rTMS treatment, including relieving of tissue edema, reducing of apoptosis and damaged cells, decreasing of synaptic and neuropil lesions, and alleviating of angioedema. Conclusion：TMS showed neural protective effects in TLE,and can relieve the pathological damage in the hippocampal ultrastructure caused by TLE.

关 键 词：经颅磁刺激 癫 颞叶 疾病模型 动物 海马 显微镜检查 电子 透射 大鼠 Wistar 

分 类 号：R135.11[医药卫生—劳动卫生]