G蛋白在快速老化痴呆小鼠SAMP8脑组织中的表达  

Abnormal Expression of G Protein in the Brain of Senescence-Accelerated Mouse Model

作　　者：阚伯红[1] 张海霞[1] 于建春[1] 韩景献[1] 

机构地区：[1]天津中医药大学第一附属医院分子生物学实验室、天津市针灸研究所,300193

出　　处：《天津医药》2010年第11期980-982,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30772839,30873302);天津市应用基础及前沿技术研究计划重点项目(项目编号:08JCZDJC15800)

摘　　要：目的:观察在不同月龄小鼠SAMP8皮质和海马中G蛋白的表达情况。方法:利用Western-blot方法检测Gαq/11、Gαi和GαS蛋白在8月龄和18月龄快速老化痴呆小鼠SAMP8的表达,并以正常老化小鼠SAMR1作为对照研究。结果:与同月龄SAMR1相比,Gαq/11、Gαi和Gαs蛋白在8月龄小鼠SAMP8皮质和海马组织中的表达无明显异常(P>0.05),而到18月龄时,SAMP8皮质部位Gαq/11表达量低于SAMR1(P<0.05),海马部位Gαq/11和Gαi的表达量低于SAMR1(P<0.05)。结论:18月龄SAMP8脑组织中Gαq/11和Gαi的异常表达参与了阿尔茨海默病(AD)的病理进展,并有望作为AD治疗的一个靶点。Objective： To explore the expression of G protein in the cortex and hippocampus of 8-month-old and 18-month-old senescence-accelerated mouse prone 8 （SAMP8）. Methods： Expressions of Gαq/11, Gαi and GαS protein were detected in 8-month-old and 18-month-old SAMP8 by using Western-blot method. The age-matched senescence-accelerated mice resistant 1 （SAMR1） mice were as control. Results： Compared with age-matched SAMR1, there was no difference in the expressions of GαS, Gαi and Gαq/11 protein in the cortex and hippocampus of 8-month-old SAMP8 （P 0.05）. The level of Gαq/11 expression was significantly lower in cortex of SAMP8 than that of SAMR1 （P 0.05）. The levels of Gαq/11 and Gαi expression were significantly lower in hippocampus of SAMP8 than those of SAMR1（P 0.05）. Conclusion： Abnormal expressions of Gαq/11 and Gαi involved in the pathological progress of Alzheimer disease （AD） in 18-month-old SAMP8, which were expected to be a target for AD treatment.

关 键 词：阿尔茨海默病 GTP结合蛋白质类 印迹法 蛋白质 小鼠 模型 动物 

分 类 号：R973[医药卫生—药品]