油蟠桃组合遗传连锁图谱构建及糖酸性状QTL分析  

Mapping QTLs Controlling Flavor Quality of Peach Using a Cross Population Between Nectarine and Flat Nectarine

作　　者：沈志军[1] 马瑞娟[1] 俞明亮[1] 蔡志翔[1] 许建兰[1] 

机构地区：[1]江苏省农业科学院园艺研究所,南京210014

出　　处：《园艺学报》2010年第11期1735-1744,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30871681);国家科技支撑计划项目(2006BAD01A1702;2008BAD92B02);江苏省科技支撑计划项目(BE2008367);江苏省自然科学基金项目(BK2004430);江苏省科技基础设施建设计划项目(BM2008008)

摘　　要：以油桃和油蟠桃组合‘霞光’בNF’杂交群体115个单株为试材,使用高效液相色谱测定亲本及各单株果实糖和有机酸含量,筛选164对SSR引物和2304对SRAP引物组合,选择符合1︰1分离比例的标记,使用Map Manager QTX20b软件的BC1模型构建遗传图谱,并对8个符合正态分布的性状进行与标记间的回归分析以及QTL区间分析。构建的图谱经与李属SSR参考图谱比对,发现基本匹配的8个连锁群总长度为1232.7cM,位点间的平均遗传距离为9.34cM,包含22个SSR标记和108个SRAP标记,以及扁平果形(S)和非酸(D)两个位点。QTL区间扫描发现,LOD≥2的QTL区间共有28个,LOD≥3的有17个,LOD≥5的有10个。风味相关的QTL分布在Group1、2、4、5、6连锁群上。Group1含4个QTL区间(Glu2、Sor2、Tsug4、SSC3);Group2含3个QTL区间(Glu4、Sor3、SSC4);Group4含5个QTL区间(Suc2、Glu3、Glu5、Tsug5、Taci4);Group1、2、4的QTL均对相应性状均表现为负加性效应;Group5含6个QTL区间(Suc1、Suc3、Tsug3、Mal1、Taci3、Taci5、),大多集中在非酸位点(D)附近,均表现为对糖含量的正加性效应,对酸含量的负加性效应;Group6含9个QTL区间(Glu1、Fru1、Sor1、Tsug1、Tsug2、Taci1、Taci2、SSC1、SSC2),所有QTL均对相应性状呈正加性效应。Flat nectarine with excellent fruit quality was one of the important objectives of peach breeding currently.A progeny with 115 individuals from a cross of nectarine and flat nectarin（eXiaguang × NF） was used as plant materials to construct genetic linkage map and to scan QTLs controlling fruit flavor.Sugars and organic acids,including sucrose,glucose,sorbitol,fructose,malic acid,citric acid and quinic acid,were determined by HPLC.A total of 213 SSR and SRAP loci,which fit the 1：1 Mendelian expectation,were selected for the construction of linkage map by MapManager QTX20b.By comparing to SSR loci from T × E reference map of Prunus,eight linkage groups were found to be matched well.A 1 232.7 cM linkage map was formed with an average distance of 9.34 cM between two loci,and the map contains 22 SSR markers,108 SRAP markers and two quality traits,flat shape（S）and non acid（D）.Through interval mapping,28,17,and 10 QTLs were found when LOD≥2,LOD≥3,and LOD≥5,respectively.QTLs controlling fruit flavor were located on Group 1,Group 2,Group 4,Group 5 and Group 6.QTLs in Goup 1（Glu2,Sor2,Tsug4 and SSC3）,Group 2（Glu4,Sor3 and SSC4）and Group 4（Suc2,Glu3,Glu5,Tsug5 and Taci4）were all showed to have a negative adding effects to their corresponding traits.Six QTLs located in Group 5（Suc1,Suc3,Tsug3,Mal1,Taci3,and Taci5）which were mainly around the non-acid loci（D）,showed a positive adding effects to sugar,and a negative adding effects to organic acid.Nine QTLs were found on Group 6（Glu1,Fru1,Sor1,Tsug1,Tsug2,Qui1,Taci1,Taci2,SSC1 and SSC2）,and all of them performed to have a positive adding effects to their corresponding traits.

关 键 词：桃 SSR SRAP 连锁图谱 糖 有机酸 QTLS 

分 类 号：S662.1[农业科学—果树学]