油松成熟胚培养直接器官发生与植株再生(英文)  

Direct Organogenesis and Plantlet Regeneration from Mature Zygotic Embryos of Chinese Pine(Pinus tabulaeformis Carr.)

作　　者：孔冬梅[1] 万婷[1] 

机构地区：[1]山西大学生命科学学院,太原030006

出　　处：《植物研究》2010年第6期668-673,共6页Bulletin of Botanical Research

基　　金：The Scientific and Technological Foundation for Young Scientists of Shanxi Province(2008021042)

摘　　要：以油松（Pinus tabulaeform is Carr.）成熟合子胚为外植体进行了直接器官发生研究。在添加15 mg.L^-1BA,或再配合添加110 mg.L^-12,4-D的DCR和MS培养基上均能诱导发生不定芽。在只添加1 mg.L^-1BA的DCR培养基上不定芽诱导率最高,为90%。及时将不定芽继代于不含生长调节物质的DCR培养基上有利于芽的伸长。添加1 mg.L^-1IBA的1/2DCR培养基最适于不定根的诱导,在该培养基上不定根诱导率达到47.1%。再生植株在蛭石基质上炼苗后转到温室培养,成活率高达86.4%,且生长正常。Mature zygotic embryos of Chinese pine were cultured as initial explants to investigate the process of direct organogenesis.Adventitious buds were initiated on DCR and MS medium supplemented with 15 mg·L-1 benzyladenine（BA） with or without 110 mg·L-1 2,4-dichlorophenoxyacetic acid（2,4-D）.The highest induction frequency of adventitious buds（90%） was obtained on DCR medium containing 1 mg·L-1 BA.Subsequent transfer of buds to plant growth regulator（PGR）-free medium in time was advantageous for elongation of adventitious buds.1/2DCR medium with 1 mg·L-1 indole-3-butyric acid（IBA） was suitable for adventitious root initiation,on which average rooting frequency was 47.1%.Plantlets were successfully acclimatized in vermiculite and transferred to greenhouse,where they had a survival rate of 86.4% and no morphological abnormalities.

关 键 词：油松 微繁 不定芽 生根 炼苗 

分 类 号：S718.46[农业科学—林学]