He-Ne激光对小麦ROP GTPase经增强UV-B辐射造成的损伤的修复研究  

Renovation of ROP GTPase in Young Seedlings of Wheats by He-Ne Laser under Enhanced UV-B Radiation

作　　者：安旭亮[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,临汾041000

出　　处：《植物研究》2010年第6期725-730,共6页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(30671061);山西省自然科学基金项目(20041101)

摘　　要：采用He-Ne激光辐照对增强UV-B辐射后小麦幼苗ROP GTPase损伤修复作用进行了研究。采用了SDS-PAGE电泳法检测各组ROP GTPase的含量和激光共聚焦显微镜对小麦微丝进行FITC荧光强度标记的测定。研究结果表明:经增强UV-B辐射后,小麦幼苗的Rop GTPase含量降低,LCSM扫描细胞原生质体形状发生改变,细胞骨架受到破坏,其被标记的荧光变暗,强度减弱,在整个UV-B处理期间均低于对照组(CK),再以He-Ne激光处理后,其含量,形状和强度均有所提高,但仍低于对照组。由此说明,UV-B辐射能使小麦幼苗的Rop GTPase含量下降,微丝骨架受到破坏,一定剂量的激光对UV-B辐射后小麦的蛋白含量和细胞骨架有一定修复作用。Rop GTPase参与了微丝骨架重组的过程。Using He-Ne laser irradiation to repair the ROP GTPase damage by the enhanced UV-B radiation in wheat seedlings has been studied.We extracted small G protein,and then determined its content by SDS-PAGE electrophoresis.At the same time,we tested the FITC fluorescence intensity of wheat microfilament marker by using a laser scanning confocal microscope.The results showed that,the enhanced UV-B radiation reduced the Rop protein content in wheat seedlings,and we also found that it changed the shape of protoplast by LCSM scanning.The intensity of the fluorescence which marks damaged cytoskeletal by the enhanced UV-B radiation was weaken.Moreover,in the whole period of UV-B treatment,the fluorescence degree was lower than the control group（CK）.Then,after treating with the He-Ne laser,the content,the shape and the intensity were all increased,but still lower than the control guoup.From the above descriptions,we can say that the UV-B radiation could decrease the Rop GTPase content of wheat seedlings and damage microfilament cytoskeleton.However,a fixed dose of laser played a role in repairing the low protein content and the cell cytoskeleton damaged by the UV-B radiation.By the way,the Rop proteins participate in the reorganization of the actin cytoskeleton.

关 键 词：He—Ne激光 ROP GTPASE SDS—PAGE电泳 UV—B辐射 小麦幼苗 

分 类 号：S512.1[农业科学—作物学]