防己抑藻效应及其化感物质的HPLC分析  

Inhibitory Effect of Algae Growth and HPLC Analysis of Allelochemicals from Stephania tetrandra

作　　者：柴民伟[1] 石福臣[1] 马妍[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《植物研究》2010年第6期758-762,共5页Bulletin of Botanical Research

基　　金：高等学校博士学科点专项科研基金(No.20050055041)

摘　　要：通过常温水浸提方法获得12种药用植物浸提液,并分别研究其抑藻效应。结果显示:防己水浸提物(相对浓度1 g.L-1)抑制蛋白核小球藻的效应最强,最大比生长率为-0.28 d-1。防己甲醇浸提物对蛋白核小球藻的抑藻效应显著,且持续时间长,最低有效抑藻浓度为30 mg.L-1。HPLC鉴定结果显示:防己水浸提液和甲醇浸提液中主要含有防己诺林碱、粉防己碱等生物碱类化学成分,防己对治理水华发生有较大的应用前景。Aqueous extracts of 12 kinds of medicinal plants were obtained by soak extraction,and their inhibitory effects on algae growth were studied.The results showed that： when the relative concentration was 1 g·L-1,aqueous extracts of Stephania tetrandra had the strongest inhibitory effect on the growth of Chlorella pyrenoidosa,and the maximum specific growth rate was-0.28 d-1.The methanol extract of S.tetrandra had significant and long-lasting inhibitory effect on the growth of C.pyrenoidosa and the lowest effective concentration in inhibiting algae was 30 mg·L-1.The identification results of HPLC showed that： the main constituents in the extracts were alkaloids such as fangchinoline and tetrandrine,etc.S.tetrandra had great prospect of application in controlling water bloom.

关 键 词：药用植物 水华 防已 蛋白核小球藻 高效液相色谱 

分 类 号：X506[环境科学与工程—环境工程]