犀角地黄汤合银翘散含药血清抑制流感病毒感染大鼠肺泡巨噬细胞致炎物质的研究  

Study on effects of serum containing Xijiao Dihuang and Yinqiaosan Decoction on inflammatory substances in alveolar macrophages against influenza virus infection

作　　者：吴莹[1] 金叶智[1] 孟建 吴珺[1] 于雪飞[1] 郝钰[1] 

机构地区：[1]北京中医药大学中医药防治病毒性疾病教育部重点实验室,北京100029 [2]北京市王府中西医结合医院,北京102209

出　　处：《中华中医药杂志》2010年第12期1974-1978,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30772871)~~

摘　　要：目的:研究犀角地黄汤合银翘散含药血清对甲型流感病毒鼠肺适应株(FM1)感染大鼠肺泡巨噬细胞(NR8383)后产生的致炎物质的影响。方法:运用中药血清药理学的方法制备等效剂量(13.8g/kg)的大鼠犀角地黄汤合银翘散含药血清。FM1感染NR8383细胞1h后,加入15%的犀角地黄汤合银翘散含药血清,同时设正常细胞和病毒对照组。药物作用后6、12、24h,ELISA检测细胞上清中炎症细胞因子TNF-α、MCP-1的含量,放免法检测细胞上清中炎性介质PGE2、PLA2、LTB4的含量;药物作用后8、24、36、48h,生化法检测细胞内自由基NO、iNOS含量;药物作用后24h,RealtimePCR检测细胞内TNF-α、MCP-1的mRNA水平。结果:犀角地黄汤合银翘散含药血清抑制了FM1感染NR8383细胞后TNF-α、MCP-1的转录和表达(P<0.05),抑制了NO、iNOS的产生(P<0.05),降低了PGE2、PLA2、LTB4、MDA的含量(P<0.05)。结论:犀角地黄汤合银翘散含药血清明显抑制了流感病毒感染后肺泡巨噬细胞内炎性细胞因子、炎性介质及自由基的产生,具有抗炎作用。Objective： To study the effects of serum containing Xijiao Dihuang and Yinqiao san Decoction on inflammatory substances generated from rat alveolar macrophage （NR8383） against infection of influenza virus A, adapted to the mice （FM1）. Methods： Serum pharmacodynamics method was used to prepare serum containing equivalent dosage （13.8g/kg） Xijiao Dihuang and Yinqiaosan Decoction. After 1 h adsorption of FM1, the medium with 15% serum containing Xijiao Dihuang and Yinqiaosan Decoction was added to NR8383 cells in wells. The normal cell group as well as virus group were prepared as controls. At 6 h, 12 h, 24 h, ELISA method was used to detect the concentration of tumor necrosis factor-alpha （TNF-α） and monocyte-specific chemoattractant molecules （MCP）-1 in the supernants, and radioimmunoassay （RIA） was used to detect the concentration of inflammatory mediators, Prostaglandin E2 （PGE2）, Phospholipase A2 （PLA2）, and leukotriene （LTB4）; at 8 h, 24 h,36 h, 48 h after drug application, biochemical detection was used to measure the concentration of NO and iNOS; at 24 h, realtime PCR was used to detect the mRNA level of TNF-α and MCP-1. Results： Xijiao Dibuang and Yinqiaosan Decoction inhibited transcription and expression of TNF-α, MCP-1 in NR8383 cells against influenza virus infection （P〈0.05）, reduced the production of oxygen free radicals NO, iNOS （P〈0.05）, and reduced the concentration of PGE2, PLA2 and LTB4 （P〈0.05）. Conclusions： Xijiao Dthuang and Yinqiaosan Decoction obviously inhibited the production of inflammatory cytokines, inflammatory mediators and oxygen flee radical in alveolar macrophage against influenza virus infection, and had anti-inflammatory effects in influenza virus infection.

关 键 词：犀角地黄汤合银翘散 含药血清 流感病毒 肺泡巨噬细胞 致炎物质 

分 类 号：R285[医药卫生—中药学]