电针预先介入对痛经模型大鼠子宫微循环的影响  

Experimental study on uterus microcirculation of preventive electroacupuncture on dysmenorrhea rat model

作　　者：李春华[1] 嵇波[1] 赵雅芳[1] 任晓暄[1] 郭孟玮[1] 丁喜艳[1] 齐丛会[1] 张露芬[1] 朱江[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《中华中医药杂志》2010年第12期1979-1983,I0001,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)课题(No.2006CB504503);国家中医药管理局针灸特色疗法评价重点研究室;国家中医药管理局针灸生物学三级实验室~~

摘　　要：目的:观察电针预先介入对痛经模型大鼠子宫微循环的影响,进一步探讨针刺缓解胞宫疼痛的作用机制。方法:将动情间期雌性大鼠随机分为盐水组、模型组、三阴交组、悬钟组、非穴组,每组6只。除盐水组外,其余各组大鼠均连续10d给予皮下注射苯甲酸雌二醇注射液,末次给药1h后,腹腔注射缩宫素2u/只,制备痛经大鼠模型,盐水组每日给予同等剂量的生理盐水。于第8d各治疗组给予电针20min,盐水组及模型组束缚20min,每日1次,连续3d,于第10d,采用XW-B-3冷光源微循环显微检查仪观察各组大鼠子宫微循环不同时间段变化。结果:与模型组比,电针三阴交穴各时间段(5、10、20min)微血管管径、cap管径(除5min外)明显扩张,微血管条数、cap条数(除5、10min外)明显增多,血流明显加快(P<0.01,P<0.05,P<0.01,P<0.05,电针悬钟穴各时间段微血管管径明显扩张、微血管条数明显增多P<0.05,P<0.01,P<0.05,电针悬钟穴、非穴20min时cap管径明显扩张P<0.01,P<0.05)。结论:电针预先介入不同穴位可缓解子宫血管的痉挛状态,改善子宫微循环,缓解疼痛,且穴位之间存在差异,三阴交>悬钟>非穴。Objective： To observe effects of uterine rnicrocirculation of preventive electroacu-puncture on dysmenorrhea rat model, to discuss the mechanism of acupuncture in relieving uterine pain. Methods： Diestrus female rats were randomly divided into model group, saline group, Sanyinjiao （SP6） group, Xuanzhong （GB39） group, non-acupoint group, each group had 6 rats. Estradiol benzoate and oxytocin were chosen to be the model of dysmenorrhea. Except saline group, other groups were given subcutaneous estradiol benzoate injection, lh after the last injection, intra-peritoneal injection of oxytocin 2u/each, Saline group given the same dose of saline per day, On the 8th day, treatment groups were given electro-acupuncture for 20 minutes, saline group and model group were bounded for 20 minutes, once a day, for3 days. On the 10th day, using XW-B-3 Cold Light micro- circulation tester observe the changes of uterine mierocirculation at different times. Results： Compared with model group, electro- acupuncture （EA） on SP6 at various time （5, 10, 20min）, diameters of micro-vessel and cap （except 5min） were significantly expanded, numbers of micro-vessel and cap （except5, 10rain） were significantly increased, blood flow was speed up significantly, differences were significant, P〈0.01, P〈0.05, P〈0.01, P〈0.05, EA on GB39 at various time, diameters of micro-vessel were significantly expanded, numbers of micro-vessel were significantly increased, differences were significant, P〈0.05, P〈0.01, P〈0.05, EA on GB39 and non-acupoint at 20min, diameters of cap were significantly expanded, P〈0.01, P〈0.05. Conclusion： Preventive electro-acupuncture can relieve spasm of uterine blood vessels, improve micro-circulation and relieve pain, there are differences among acupoints, SP6〉GB39 〉non-acupoint.

关 键 词：痛经 子宫微循环 穴位 非穴 

分 类 号：R245[医药卫生—针灸推拿学]