针刀松解法对第三腰椎横突综合征兔炎性细胞因子影响的研究  

Effect of acupotomy lysis on inflammatory cytokine in serum and muscle in rabbit with the third lumbar vertebrae transverse process syndrome

作　　者：李晓泓[1] 刘乃刚[1] 孙红梅[1] 郭长青[1] 李宰和[1] 孔珉晶[1] 张义[1] 吴海霞[1] 许红[1] 岳利锋[1] 乔晋琳[2] 陈占禄[1] 

机构地区：[1]北京中医药大学,北京100029 [2]解放军海军总医院康复理疗科,北京100037

出　　处：《中华中医药杂志》2010年第12期2163-2166,共4页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:观察第三腰椎横突综合征模型兔血清和肌肉组织匀浆中炎性细胞因子的含量变化,探讨针刀松解法的干预作用机制。方法:将30只日本大耳白兔随机分为正常组、模型组、针刀组、电针组和针刀+电针组5组,每组6只。后4组采用在左侧第3腰椎横突部埋置明胶海绵的方法制作第3腰椎横突综合征模型,针刀组和电针组分别给予针刀松解法和电针双侧"委中"干预,针刀+电针组则同时给予针刀松解法和电针干预。造模后第28d,取腹主动脉血和左侧第3腰椎横突周围肌肉组织,采用双抗夹心ELISA方法检测血清和肌肉组织匀浆中IL-1β、IL-6、IL-8、TNF-α、IL-10、TGF-β含量的变化。结果:模型组血清和肌肉组织匀浆中IL-1β、IL-6、IL-8、TNF-α、IL-10、TGF-β含量较正常显著升高,有极显著差异(P<0.01),针刀组、电针组和针刀+电针组较模型组各炎性细胞因子的含量较模型组具有明显下降,有显著差异(P<0.05,P<0.01)。结论:针刀松解法可明显降低模型兔血清和肌肉组织匀浆中的各种炎性细胞因子,改善局部炎症反应,表明针刀松解法对外周各种炎性细胞因子的良性调节作用可能是其抗炎干预作用的机制之一。Objective： To observe the change of inflammatory cytokine in serum and muscle tissuehomogenate in rabbit with the third lumbar vertebrae transverse process syndrome, and study the therapeutic mechanism of acupotomy lysis. Methods： 30 Japanese white rabbits were randomly divided into normal control group, model group, acupotomy lysis （AL） group, electroacupunture （EA） group and AL＋EA group. The model in rabbits with the third lumbar vertebrae transverse process syndrome was established by inserting a piece of gelatin sponge into the posterior of the left 3rd lumbar vertebrae transverse process. AL group and EA group are used acupotomy lysis and EA therapy applied to ＂Wei Zhong＂ of both sides, while AL＋EA group used the two treatments. IL-1β, IL-6, IL-8, TNF-α, IL-10, TGF-β contents in serum and muscle tissuehomogenate in rabbits were tested by Double Sandwich enzyme-linked immunoab sorbant assay （ELISA） technology on day 28. Results： In model group, IL-1β, IL-6, IL-8, TNF-α, IL-10, TGF-β contents in serum and muscle tissuehomogenate was increased significantly than normal （P〈0.01）, while the contents of AL group , EA group and AL＋EA group were decreased than model group significantly （P〈0.05, P〈0.01）. Conclusion： Acupotomy lysis can decrease the contents of the inflammatory cytokine in serum and muscle tissuehomogenate, reduce the inflammatory response, and indicate that the regulating action on inflammatory cytokine was one of the intervention mechanism of Acupotomy lysis.

关 键 词：针刀松解法 第3腰椎横突综合征 炎性细胞因子 兔 

分 类 号：R245[医药卫生—针灸推拿学]