清开灵注射液治疗局灶性脑缺血再灌注模型的时间窗研究  

Study on the Therapeutic time window of Qingkailing’s neuroprotective effect in a mouse model of FCIR

作　　者：程发峰[1] 郭少英[1] 钟相根[1] 鲁艺[1] 王庆国[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药杂志》2010年第12期2207-2209,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：重大新药创制科技重大专项(No.2009ZX09102-136)~~

摘　　要：目的:研究清开灵注射液对局灶性脑缺血再灌注损伤的治疗时间窗。方法:线栓法制备昆明小鼠局灶性脑缺血再灌注损伤模型,分别在缺血后0、1、3、4、6h经尾静脉给予清开灵注射液,24h后进行神经功能评分,处死后取脑,TTC染色并计算脑梗塞体积。结果:各给药组均能显著减少梗塞体积,各组局灶功能损伤均有显著改善,一般功能损伤和Bederson评分只有0h给药组有显著改善。结论:清开灵注射液可减少局灶性脑缺血再灌注损伤小鼠的脑梗塞体积并改善其神经功能,其治疗时间窗可达到6h。Objective To study the protection and therapy time window of QKL injection on mice models of focal cerebral ischemia.Methods：Seventy Kunming mice were randomly and evenly divided into groups.Cerebral ischemia model of middle cerebral artery occlusion was established in mice using the suture method.Neurological deficit evaluations,such as Bederson,Clark scoring methods,were then carried out at 24 hours after MCAO and cerebral infarction volume were examined after the TTC staining.Results：The results showed that cerebral infarction volume reduced significantly after QKL injection administration at time 0、1、3、4、6h.Local cerebral injury recovered significantly,however there was no obvious difference between model group and other groups on general function injury or Bederson score except the 0h administration group.Conclusion：Qingkailing injection is a promising therapeutic strategy for cerebral ischemia and its therapeutic time window could last for 6 hours after the focal cerebral ischemia reperfusion,which would help in lessening the ischemic brain damage and improve neurological function.

关 键 词：局灶性脑缺血 清开灵注射液 时间窗 脑梗塞体积 神经功能评分 

分 类 号：R285.5[医药卫生—中药学]