Microfilament Dynamics is Required for Root Growth under Alkaline Stress in Arabidopsis  

作　　者：Yue Zhou Zijun Yang Guangqin Guo Yan Guo 

机构地区：[1]Institute of Cell Biology,School of Life Sciences,Lanzhou University,Lanzhou 730000,China [2]National Institute of Biological Sciences,Beijing,7 Science Park Road,Zhongguancun Life Science Park,Beijing 102206,China [3]Agricultural Technology Center,Huadian 132400,China

出　　处：《Journal of Integrative Plant Biology》2010年第11期952-958,共7页植物学报（英文版）

基　　金：supported by the State Key Basic Research and Development Program of China (2006CB100100);the National High Technology Research and Development Program of China (863) (2003AA210100) to Y.G

摘　　要：The microfilament （MF） cytoskeleton has crucial functions in plant development.Recent studies have revealed the function of MFs in diverse stress response.Alkaline stress is harmful to plant growth;however,it remains unclear whether the MFs play a role in alkaline stress.In the present study,we find that blocking MF assembly with latrunculin B （Lat B） leads to inhibition of plant root growth,and stabilization of MFs with phalloidin does not significantly affect plant root growth under normal conditions.In high external pH conditions,MF de-polymerization is induced and that associates with the reduction of root growth;phalloidin treatment partially rescues this reduction.Moreover,Lat B treatment further decreases the survival rate of seedlings growing in high external pH conditions.However,a high external pH （8.0） does not affect MF stability in vitro.Taken together,our results suggest that alkaline stress may trigger a signal that leads the dynamics of MFs and in turn regulates root growth.The microfilament （MF） cytoskeleton has crucial functions in plant development.Recent studies have revealed the function of MFs in diverse stress response.Alkaline stress is harmful to plant growth;however,it remains unclear whether the MFs play a role in alkaline stress.In the present study,we find that blocking MF assembly with latrunculin B （Lat B） leads to inhibition of plant root growth,and stabilization of MFs with phalloidin does not significantly affect plant root growth under normal conditions.In high external pH conditions,MF de-polymerization is induced and that associates with the reduction of root growth;phalloidin treatment partially rescues this reduction.Moreover,Lat B treatment further decreases the survival rate of seedlings growing in high external pH conditions.However,a high external pH （8.0） does not affect MF stability in vitro.Taken together,our results suggest that alkaline stress may trigger a signal that leads the dynamics of MFs and in turn regulates root growth.

分 类 号：Q245[生物学—细胞生物学] S512.11[农业科学—作物学]