冰箱保藏双孢蘑菇菌株的遗传特性研究  

A Study on the Genetic Characteristics of A . bisporus Strains Stored in the Refrigerator

作　　者：凌霞芬[1] 郭倩 詹才新 贺冬梅[1] 冯志勇[1] 谭琦[1] 陈明杰[1] 潘迎捷[1] 张引芳 高洪亮 

机构地区：[1]农业部食用菌遗传育种重点开放实验室

出　　处：《食用菌学报》1999年第2期15-19,共5页Acta Edulis Fungi

摘　　要：从菌落形态、生理生化特性和DNA水平等方面研究双孢蘑菇菌株于4℃冰箱保存后的遗传特性。结果表明,冰箱保存菌株的活力普遍下降;部分菌株的菌落形态发生变化,酯酶同工酶也存在一些差异;但RAPD分析表明,冰箱保存菌株与生产栽培菌株无明显的遗传差异。A study of the colonial morphology, the esterase isozyme zymogram and the random amplified polymorphic DNA(RAPD) of A. bisporus strains stored in the refrigerator at 4t was made. The result showed that the vigour of all the strains stored in the refrigerator was reduced generally, that the colonial morphology and the PAGE zymogram of esterase isozyme of some refrigerator - stored strains were changed a little, and that no significant genetic difference was found between the refrigerator - stored strains and the commercial strains through RAPD analysis.

关 键 词：双孢蘑菇 RAPD 冰箱保存 酯酶同工酶 遗传特性 

分 类 号：S646.110.3[农业科学—蔬菜学]