电针不同经穴对直-结肠扩张大鼠血压及自主神经系统的影响  

Effects of Electroacupuncture of Different Acupoints on Changes of Blood Pressure and Autonomic Nerve System after Colorectal Distension in Rats

作　　者：陈淑萍[1] 高永辉[1] 喻晓春[2] 刘俊岭[1] 

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]中国中医科学院医学实验中心,北京100700

出　　处：《针刺研究》2010年第5期335-341,共7页Acupuncture Research

基　　金：国家重点基础研究发展计划中医专项(No.2005CB523306;2006CB504506;2007CB512505);国家自然科学基金项目(No.30472241;90709031;30973796)

摘　　要：目的：观察电针对直-结肠扩张（CRD）大鼠血压、心率（HR）及心率变异性（HRV）变化的影响,比较不同穴位对内脏痛、自主神经系统变化调节效应的相对特异性。方法：45只Wistar大鼠,随机分为对照组、足三里组、非穴组、太冲组及内关组,每组9只。麻醉状态下,用肛门内气囊扩张（压力为50~60 mmHg）大鼠直-结肠5 min,电针＂足三里＂＂太冲＂＂内关＂及非穴区各15 min。电生理记录仪记录颈-胸导联心电图、HR、颈动脉平均压（MAP）及HRV,用频域测定法分析HRV的高频（HF）、低频（LF）成分,并计算其比值。结果：CRD后,大鼠MAP、HR、LF及LF/HF均明显增加（P〈0.05）,HF变化不大（P〉0.05）。与对照组比较,电针后5、15 min及停针后10 min,足三里组及内关组MAP明显降低（P〈0.05）。电针15 min、停针10 min时内关组及停针10 min时足三里组的HR明显低于同组各自CRD期间的值（P〈0.05）。电针5 min时足三里及内关组,15 min时足三里、内关、太冲组,停针10 min时足三里组的LF均明显低于对照组（P〈0.05）。停针10 min时足三里及内关组的LF/HF明显低于对照组（P〈0.05）。各电针组之间的HR、LF及LF/HF值差异均无统计学意义（P〉0.05）。结论：电针＂足三里＂＂内关＂穴可显著降低大鼠CRD后异常升高的MAP、HR及LF/HF,提示电针该两穴区可明显缓解内脏痛,良性调节CRD引起的自主神经功能紊乱,而＂太冲＂和后肢非穴区作用不明显。Objective To observe the influence of electroacupuncture（EA） of different acupoints on changes of mean arterial pressure（MAP）,heart rate（HR） and heart rate variability（HRV） in colorectal distension（CRD） rats,so as to analyze the specificity of actions of acupoints in relieving visceral pain and regulating activities of the autonomic nerve system.MethodsForty-five Wistar rats were randomized into control,Zusanli（ST 36）,non-acupoint,Neiguan（PC 6） and Taichong（LR 3） groups（n=9/group）.Under anesthesia,CRD was given to the rats by using an aerostat for 5 min.EA（2 Hz/15 Hz,2 mA） was applied to bilateral ST 36,non-acupoint（1.0 cm lateral to ST 36）,PC 6 and LR 3 for 15 min,respectively.Electrocardiogram of the cervico-chest lead was recorded by using a bioelectric amplifier,and MAP recorded by using a pressure transducer and an amplifier.Low frequency and high frequency of HRV were analyzed by Chart 5.0.Results Following CRD,the HR,MAP,LF and LF/HF levels increased significantly in all the 5 groups（P0.05）,while HF had no apparent changes（P0.05）.Compared with the control group,5 min and 15 min after EA,and 10 min after ceasing EA,MAP values of ST 36 and PC 6 groups were decreased obviously（P0.05）.The HR values of ST 36 and PC 6 groups at 15 min after EA and 10 min after ceasing EA were decreased markedly in comparison with those of each of the other groups during CRD（P0.05）.The LF levels of both ST 36 and PC 6 groups at 5 min after EA,and those of ST 36,PC 6 and LR 3 at 15 min after EA were significantly lower than those of control group（P0.05）.LF/HF levels of ST 36 and PC 6 at 10 min after ceasing EA were obviously lower than those of control group（P0.05）.No significant differences were found among ST 36,non-acupoint,PC 6 and LR 3 groups in HR,LF and LF/HF（P0.05）.Conclusion EA of ST 36 and PC 6 can suppress CRD-induced increase of MAP,HR and LF/HF,suggesting beneficial effects of EA in relieving visceral pain and mediating autonomic nerve system.The

关 键 词：直-结肠扩张 心率变异性 平均动脉压 电针 足三里 太冲 内关 

分 类 号：R245.97[医药卫生—针灸推拿学]