针刀松解法对第3腰椎横突综合征大鼠下丘脑前阿黑皮素mRNA、前脑啡肽原mRNA表达的影响  

Effect of Acupotomy Lysis on Hypothalamic POMC mRNA and PPE mRNA Expression in Rats with 3^(rd) Lumbar Vertebrae Transverse Process Syndrome

作　　者：郭长青[1] 曹榕娟[1] 孙红梅[2] Seyed Javad Mojtabavi 马惠芳[1] 吴海霞[2] 许红[2] 李晓泓[1] 刘乃刚[1] 张义[1] 岳利锋[2] 乔晋琳[3] 陈占禄[4] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]北京中医药大学基础医学院,北京100029 [3]解放军海军总医院康复理疗科,北京100037 [4]北京中医药大学管理学院,北京100029

出　　处：《针刺研究》2010年第5期354-358,393,共6页Acupuncture Research

基　　金：国家重点基础研究发展计划"973"计划(No.2006CB504508);国家中医药管理局<针灸特色疗法评价>重点研究室资助;教育部<高等学校本科教学质量与教学改革工程>高等学校专业建设点项目北京市教育委员会共建项目

摘　　要：目的:探讨针刀松解法治疗第3腰椎横突综合征的部分中枢镇痛机制。方法:SD雄性大鼠24只,随机分为正常组、模型组、针刀组及电针组。采用左侧第3腰椎横突尖部埋置明胶海绵法建立第3腰椎横突综合征模型,针刀组和电针组分别给予针刀松解和电针左侧"肾俞""腰阳关"治疗。应用原位杂交方法检测大鼠下丘脑前阿黑皮素(POMC)mRNA、前脑啡肽原(PPE)mRNA阳性细胞的表达。结果:造模后大鼠下丘脑POMC mRNA、PPE mRNA的阳性细胞表达较正常组增多(P<0.01),针刀组和电针组大鼠下丘脑POMC mRNA、PPE mRNA阳性细胞表达较模型组进一步增多(P<0.01)。结论:针刀松解法与电针可能通过促进下丘脑POMC mRNA、PPE mRNA的表达,参与其镇痛过程。Objective To observe the effect of acupotomy lysis（AL） on hypothalamic proopiomelanocortin（POMC） mRNA and preproenkephalin（PPE） mRNA expression in rats with the third lumbar vertebrae transverse process syndrome（TLVTPS） so as to study its underlying mechanism in relieving symptoms of lumbar muscle strain.Methods Twenty-four SD rats were randomly divided into normal control group,model group,AL group and electroacupunture（EA） group.The TLVTPS model was established by inserting a piece of gelatin sponge into the posterior of the left 3rd lumbar vertebrae transverse process.AL and EA were applied to the left ＂Shenshu＂（BL 23） and ＂Yaoyangguan＂（GV 3） respectively.The POMC mRNA and PPE mRNA expression levels in the hypothalamus were detected by in situ hybridization.Results In comparison with the normal group,the integrated optical density（IOD） values of hypothalamic POMC mRNA and PPE mRNA positive cells in the model group were increased significantly（P0.01）;while compared with the model group,those of POMC mRNA and PPE mRNA positive cells in both left and right hypothalamus were increased further considerably in both AL and EA groups（P0.01）.No significant diffe-rences were found between AL and EA groups in POMC mRNA and PPE mRNA expression levels（P0.05）.Conclusion AL and EA therapies can increase the expression of POMC mRNA and PPE mRNA in hypothalamus in rats with TLVTPS,which may contribute to its effect in relieving pain in the treatment of lumbar muscle strain.

关 键 词：第3腰椎横突综合征 针刀松解法 电针 下丘脑 前阿黑皮素 前脑啡肽原 

分 类 号：R245.319[医药卫生—针灸推拿学]