IL-23、IL-23 mRNA在皮肤移植受鼠外周血及移植物中的表达  

Expression of IL-23 and IL-23 mRNA in allograft and peripheral blood of mice subject to skin transplantation

作　　者：杜国盛 石炳毅 郑德华 宋继勇 朱志东 崔洪涛 

机构地区：[1]解放军第三0九医院器官移植中心,北京100091

出　　处：《中华器官移植杂志》2010年第11期683-687,共5页Chinese Journal of Organ Transplantation

基　　金：基金项目：全军“十一五”计划科研基金科技攻关课题（06G115）

摘　　要：目的 探讨皮肤移植小鼠外周血及移植物中白细胞介素23（IL-23）和IL-23 mRNA的表达情况.方法 制作小鼠皮肤移植的动物模型：同系组供、受者均为Balb/c小鼠;同种组以C57BL/6小鼠为供者,Balb/c小鼠为受者;脾细胞组以C57BL/6小鼠为供者,Balb/c小鼠为受者,受鼠移植前1 d经尾静脉输注供者脾细胞1×107个/只.移植术后1、3、5和7 d留取受者血液和皮肤移植物样本,检测血清IL-23的浓度以及移植皮肤中IL-23 mRNA的表达,并于光镜下持续观察移植皮肤的病理学变化.结果 移植后第3和5天,同系组受鼠外周血IL-23浓度分别为（263.49±18.72）和（286.55±18.95）pg/ml,同种组分别为（295.66±16.36）和（332.58±17.37）pg/ml,脾细胞组分别为（303.66±16.99）和（323.96±18.99）pg/ml.移植后第3和5天,同系组受鼠外周血IL-23浓度低于同种组和脾细胞组（P＜0.01）;同种组和脾细胞组间的差异无统计学意义（P＞0.05）.皮肤移植后第3、5和7天,同系组受鼠移植皮肤IL-23 mRNA的相对表达量分别为0.57±0.10、0.63±0.10和0.66±0.11,同种组分别为0.78±0.09、0.81±0.09和0.69±0.14,脾细胞组分别为0.62±0.10、0.68±0.12和0.55±0.09.移植后第3和5天,同系组和脾细胞组受鼠移植皮肤IL-23 mRNA的相对表达量低于同种组（P＜0.01）.移植后第7天,同种组与脾细胞组受鼠移植皮肤IL-23 mRNA的相对表达量的差异有统计学意义（P＜0.01）.结论 皮肤移植后早期发生急性排斥反应时,受鼠高表达IL-23和IL-23 mRNA.IL-23可作为预测排斥反应发生的观察指标.Objective To investigate the expression of IL-23 and IL-23 mRNA in allograft and peripheral blood of mice receiving skin transplantation under different immune states. Methods Mice skin allograft models were established and divided into 3 groups： synergeneic transplant group （BALB/c→BALB/c）, allogeneic transplant group （C57BL/6→BALB/c）, donor spleen cells infusion group （C57BL/6→BALB/c）. Peripheral blood plasma concentration of IL-23 was measured by ELISA. RT-PCR was used to detect the expression of IL-23 mRNA in the skin allograft. Results There was no significant difference in the IL-23 and IL-23 mRNA expression among all three groups one day after skin transplantation （P〉0. 05）. On the day 3, 5, and 7 after skin transplantation, there was significant difference in the IL-23 and IL-23 mRNA expression levels between synergeneic transplant group, donor spleen cells infusion group and allogeneic transplant group （P 〈 0. 01 ）, but there was no significant difference between synergeneic transplant group and donor spleen cells infusion group （P〉0. 05）. Conclusion The high expression levels of IL-23 and IL-23 mRNA were detected when early acute rejection took place in recipient mice. IL-23 could serve as a predictable and prognostic marker for the acute rejection. Infusion of donor spleen cells can significantly prolong the allograft survival.

关 键 词：急性排斥反应 白细胞介素23 MRNA 脾细胞 

分 类 号：R686[医药卫生—骨科学]