黄术灌肠液对腹泻型肠易激综合征大鼠结肠黏膜VIP表达的影响  

Huangshu Enema down-regulates VIP expression in the colonic mucosa of rats with diarrhea-predominant irritable bowel syndrome

作　　者：吴美玉[1] 胡团敏[1] 郑溪水[1] 

机构地区：[1]中国人民解放军第180医院消化内科,福建省泉州市362000

出　　处：《世界华人消化杂志》2010年第28期2966-2970,共5页World Chinese Journal of Digestology

基　　金：泉州市科学基金资助项目;No.2009Z8~~

摘　　要：目的:探讨中药自组方剂黄术灌肠液对腹泻型肠易激综合征(D-IBS)大鼠结肠黏膜血管活性肠肽(VIP)表达的影响及其治疗D-IBS的可能机制.方法:将48只成年♀SD大鼠随机分为正常对照组、模型组、黄术灌肠液治疗组、得舒特治疗组,每组12只,后3组采用灌服番泻叶加束缚应激法建立D-IBS模型.随机选取每组中的8只大鼠,运用免疫组织化学染色法测定各组大鼠结肠黏膜VIP的表达.结果:模型组大鼠结肠黏膜VIP免疫组织化学染色的阳性细胞数量和染色强度均明显高于正常对照组(21.06±2.69vs6.25±2.08,t=17.4216,P<0.01;2.43±0.63vs0.63±0.62,t=6.5223,P<0.01);经治疗后大鼠结肠黏膜VIP免疫组织化学染色的阳性细胞数和染色强度均明显低于模型组(t=11.6697,t=5.5119;t=6.0910,t=2.1575;均P<0.01),且黄术灌肠液治疗组较得舒特治疗组降低更为明显(10.62±2.36vs15.31±2.65,t=5.2876,P<0.01;1.25±0.58vs1.93±0.68,t=2.9272,P<0.01).结论:黄术灌肠液能够降低D-IBS大鼠结肠黏膜VIP免疫组织化学染色的阳性细胞数量和染色强度,通过调整脑-肠轴功能异常,改善D-IBS的症状,达到治疗效果.AIM：To investigate the effect of Chinese medicine Huangshu Enema （HSE） on the expression of vasoactive intestinal polypeptide （VIP） in the colonic mucosa of rats with diarrhea-predominant irritable bowel syndrome （D-IBS） and to explore possible mechanisms involved.METHODS：Forty-eight female Sprague-Dawley rats were randomly divided into normal control group,model group,HSE treatment group and dicetel treatment group （n=12 for each group）.D-IBS was induced in rats by intragastric administration of senna decoction and restraint stress.The expression of VIP in the colonic mucosa of rats was determined by immunohistochemistry.RESULTS：The number of VIP-positive cells and VIP staining intensity in the colonic mucosa of rats were significantly higher in the model group than in the normal control group （21.06±2.69 vs 6.25±2.08,t=17.4216,P0.01;2.43 ± 0.63 vs 0.63 ± 0.62,t=6.5223,P0.01）,in the model group than in the two treatment groups （t=11.6697 and 5.5119;t=6.0910 and 2.1575,respectively;both P0.01）,and in the dicetel treatment group than in the HSE treatment group （10.62±2.36 vs 15.31±2.65,t=5.2876,P0.01;1.25±0.58 vs 1.93±0.68,t=2.9272,P0.01）.CONCLUSION：HSE can reduce the number of VIP-positive cells and VIP staining intensity in the colonic mucosa of D-IBS rats and thereby exerts therapeutic effects against D-IBS.

关 键 词：黄术灌肠液 腹泻型肠易激综合征 血管活性肠肽 

分 类 号：R285.5[医药卫生—中药学]