骨髓间充质干细胞在小肠黏膜损伤小鼠体内的定植  

Mesenchymal stem cells engraftment in the injured intestine of mice

作　　者：吴巍芸[1] 陈其奎[2] 

机构地区：[1]南方医科大学珠江医院消化内科,广东省广州市510282 [2]中山大学附属第二医院消化内科,广东省广州市510120

出　　处：《世界华人消化杂志》2010年第29期3129-3133,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30670950~~

摘　　要：目的:探讨小鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)移植于小肠放射性损伤的小鼠体内后的定植情况.方法:体外培养扩增♂昆明小鼠MSCs,然后采用性别交叉移植的方法,即移植组:经尾静脉注射雄性小鼠MSCs进入小肠放射性损伤的雌性小鼠体内;对照组:注射等量无菌生理盐水.分别于第1、2、4周通过原位杂交检测Y染色体观察MSCs的定植情况.结果:移植组小鼠于移植后第1周即可检出Y染色体阳性荧光细胞的存在,呈零星散在分布,且多位于隐窝周围的黏膜下层,阳性率为19.48%±5.01%;第2周荧光细胞明显增多,阳性率为30.86%±12.14%,差异显著(P<0.05);第4周阳性率为35.92%±11.98%,差异不显著(P>0.05).结论:MSCs移植于小肠放射性损伤的小鼠体内后可以在小肠定植,且主要定植于黏膜下层.AIM：To investigate the engraftment of murine bone marrow mesenchymal stem cells（MSCs） in the irradiated intestine of mice to provide some clues about the mechanism of intestine epithelium healing and lay an experimental foundation for treatment of intestine diseases by intravenous transplantation of MSCs.METHODS：MSCs from male mice were isolated,expanded,identified,suspended in sterile normal saline（1 × 10^6 cells/mL）,and slowly infused into irradiated female mice via the tail vein.Meanwhile,a group of irradiated female mice receiving an equal volume of sterile normal saline were used as controls.For tracing male MSC residence in the intestine after intravenous transplantation,in situ hybridization（ISH） was used to detect the Sry gene on the Y chromosome.RESULTS：In the transplantation group,ISH analysis revealed the presence of male donor MSCs in the submucosa of the intestine of female mice,but not in the mucosal epithelium.At week 1 after cell transplantation,Sry-positive cells were scattered around the crypt,with a percentage of 19.48% ± 5.01%.At week 2,this percentage rose to 30.86% ± 12.14%,significantly higher than that at week 1（P 〈0.05）.However,there was no significant difference in the percentage of Sry-positive cells between at week 2 and week 4（week 4：35.95% ± 11.98%,P〉 0.05）.In the control group,no Sry-positive cells were found.CONCLUSION：After MSCs were transplanted into mice,they were attracted to and retained in the irradiated intestine and colonized in the intestinal submucosa.

关 键 词：骨髓间充质干细胞 体内定植 小肠黏膜下层 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]