内皮抑素对小鼠体内Lewis肺癌生长及淋巴管生成的影响  

Effects of endostatin on the growth and lymphangiogenesis of Lewis lung carcinoma xenograft in mice

作　　者：董晓鹏[1] 肖天辉[1] 孟令佰 贠灿华[1] 田辉[3] 

机构地区：[1]山东大学第二医院胸外科,济南250033 [2]山东省昌乐县中医院胸外科 [3]山东大学齐鲁医院胸外科

出　　处：《中华医学杂志》2010年第43期3091-3093,共3页National Medical Journal of China

基　　金：山东省自然科学基金青年基金（Q2007C12）

摘　　要：目的 研究内皮抑素对小鼠Lewis肺癌生长、淋巴管生成和淋巴结转移的影响.方法 用40只小鼠建立Lewis肺癌模型,然后分为两组,分别注射生理盐水（对照组）和重组内皮抑素endostar（治疗组）.记录并比较两组肺表面肿瘤数、肿瘤大小及淋巴结转移率,免疫组化法检测肿瘤微淋巴管密度和血管内皮生长因子-C（VEGF-C）表达,分析内皮抑素对肿瘤微淋巴管密度（MLVD）和VEGF-C表达的影响.结果 治疗组肺表面肿瘤数、肿瘤平均长径均低于治疗组,差异有统计学意义（P＜0.05）.对照组和治疗组MLVD分别为7.8±1.6、5.7±1. 6,两组分别有8例和2例见淋巴结转移,差异均有统计学意义（P＜0.05）.对照组VEGF-C表达阳性率为66.7%（12/18）,治疗组为27.8%（5/18）,差异有统计学意义（P＜0.05）.结论 内皮抑素对小鼠肺癌生长、淋巴管生成和淋巴结转移有抑制作用,这种抑制作用可能与内皮抑素对VEGF-C表达的调控有关.Objective To investigate the effects of endostar, a recombined humanized endostatin,on the growth, lymphangiogenesis and lymphatic metastasis of Lewis lung carcinoma xenograft in mice. Methods Lewis lung carcinoma （LLC） xenograft were established in C57 mice by intravenious transplantation of 1 × 106 cells. Then tumor-bearing mice were assigned into two groups： control group received caudal vein injections of 0. 2 ml of 0. 9% sodium chloride for 15 days, and treatment group received 500 μg endostar daily. Six weeks after LLC cell injection mice were sacrificed, and then tumor numbers and size were recorded. The expression of vascular endothelial growth factor-c （VEGF-C） and microlymphatic vessel density（ MLVD）were observed by immunohistochemical staining. Results Tumor number and size of control group were significantly higher than those of treatment group. The microlymphatic vessel density （MLVD） was 5.7 ± 1.6 in the treatment group, which was markedly lower than in the control mice （7. 8 ±1.6）. Two lymph node metastases were observed in treatment group, and eight in control group. Lymphatic metastases were more frequent in control group than in treatment group. Expression of VEGF-C in control group was significantly higher than that in treatment group. Conclusion Endostar significantly inhibits the growth, lymphangiogenesis and lymphatic metastasis of Lewis lung carcinoma xenografts, and the inhibitory effect is due to its ability to regulate the expression of VEGF-C of tumors in part.

关 键 词：肺肿瘤 生长抑制物 淋巴转移 

分 类 号：R686[医药卫生—骨科学]