人富血小板血浆及其细化成分对小鼠成骨细胞增殖的影响  

Effects of different components in human platelet-rich plasma on mouse osteoblast proliferation

作　　者：段建民[1] 李洪涛[1] 匡威[1] 文军[1] 菊地宽高 片山直 

机构地区：[1]广州军区广州总医院口腔科,510010 [2]日本明海大学齿学部机能保存修复学讲座

出　　处：《实用口腔医学杂志》2010年第6期716-720,共5页Journal of Practical Stomatology

基　　金：广东省科技计划项目国际合作基金资助项目(编号:2007B050200017)

摘　　要：目的:观察人富血小板血浆(PRP)及其细化成分对小鼠成骨细胞MC3T3-E1增殖的影响。方法:抽取年轻男性志愿者静脉血制备富血小板血浆,并提取洗涤血小板(WPLT)和乏血小板血浆(PPP)等细化成分,使用细胞定量测定试剂盒测定细胞增殖情况。结果:50ml/L的PRP对MC3T3-E1细胞的增殖没有明显影响,而50ml/L的WPLT显著促进了MC3T3-E1细胞的增殖,但是,这种促进增殖的作用效果会随着WPLT浓度的倍比增加而显著降低,并且当其浓度为150ml/L时反而会显著抑制细胞的增殖。另外,50ml/L的PPP对MC3T3-E1细胞的增殖有明显的抑制作用,而且倍比添加PPP会显著抑制50ml/LWPLT促进的MC3T3-E1细胞增殖。结论:人WPLT促进小鼠成骨细胞MC3T3-E1增殖的作用具有浓度特异性,PPP对MC3T3-E1的增殖有显著的抑制作用。Objective：To investigate the effects of different components in human platelet-rich plasma（PRP）on the proliferation of mouse osteoblast MC3T3-E1 cells.Methods：PRP was obtained from venous blood samples of a young man volunteer.Washed platelet（WPLT）and platelet-poor plasma（PPP）were extracted from the PRP.Cell quantification kit was applied to detect cell proliferation.Results：50 ml/L PRP had no significant effects on MC3T3-E1 cell proliferation,while 50 ml/L WPLT apparently promoted the proliferation of MC3T3-E1 cells.However this promoting effect decreased with the WPLT concentration increased.WPLT at 150 mol/L inhibited the MC3T3-E1 cell proliferation.In addition,50 ml/L PPP significantly inhibited cell proliferation,and PPP inhibited MC3T3-E1 cell proliferation promoted by 50 ml/L WPLT in a dose dependent manner.Conclusion：Human WPLT has a proliferation promoting effect on MC3T3-E1 cells.However,PPP may inhibit MC3T3-E1 cells proliferation.

关 键 词：人类 富血小板血浆 小鼠 成骨细胞 增殖 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]