锂-匹罗卡品致痫大鼠海马结构nNOS、iNOS及活化Caspase-3蛋白表达的动态变化  

Dynamic changes of nNOS,iNOS and activated Caspase-3 protein expressions in the hippocampus of rats with lithium-pilocarpine-induced epilepsy

作　　者：陈立光[1] 刘滨[2] 范海涛[2] 贾践博[2] 庞琦[2] 

机构地区：[1]山东省医学影像学研究所,济南250021 [2]山东大学附属省立医院神经外科,济南250021

出　　处：《山东大学学报（医学版）》2010年第11期50-53,共4页Journal of Shandong University:Health Sciences

基　　金：山东省卫生厅医药卫生发展计划项目(2009HZ053)

摘　　要：目的探讨大鼠癫痫持续状态(SE)后海马结构神经型一氧化氮合酶(nNOS)、诱导型一氧化氮合酶(iNOS)及活化Caspase-3蛋白表达的动态变化及其相互关系。方法采用锂-匹罗卡品腹腔注射建立大鼠SE模型,用免疫组织化学及免疫印迹法在2h、6h、3d及7d不同时相点检测大鼠海马nNOS、iNOS及活化Caspase-3蛋白的表达。结果大鼠海马nNOS的蛋白表达在SE后2h开始迅速增高,在6h时达到高峰,之后逐渐降低,但在3、7d时仍显著高于对照组(P均<0.01)。iNOS蛋白的表达在SE后6h时开始持续增高(P<0.01),并在7d时达到高峰(P<0.01)。活化Caspase-3蛋白的表达在3d开始增高(P<0.01),7d时达到高峰(P<0.01)。结论大鼠在SE后海马的nNOS、iNOS及活化Caspase-3的表达均有不同程度的增高,提示一氧化氮合酶的反应产物一氧化氮可能与癫痫发作后海马结构内的神经元受损有关,而神经元受损与凋亡之间的关系亦密不可分。Objective To explore the correlation and dynamic changes of protein expressions of nNOS,iNOS and activated Caspase-3 after status epilepticus （ SE） . Methods The animal model was established by lithium-pilocarine induction in rats. 2 h,6 h,3 d and 7 d after SE,variations of expressions of nNOS,iNOS and activated Caspase-3 proteins were determined by immunohistochemistry and immunoblot,respectively. Results Expression of the nNOS protein significantly increased 2 h after SE（ P 〈 0. 01） ,reached its peak at 6 h （ P 〈 0. 01） ,and decreased thereafter. However,expressions of the nNOS protein on 3d and 7d were still significantly higher compared with the control group （ P 〈0.01） . Expression of the iNOS protein was significantly up-regulated 6h after SE（ P 〈 0. 01） ,and reached its peak on 3 d （ P 〈 0. 01） . Expression of the activated Caspase-3 protein significantly increased 3 d after SE（ P 〈 0. 01） , and reached its peak on 7 d （ P 〈 0. 01） . Conclusion Expressions of nNOS,iNOS and activated Caspase-3 proteins are up-regulated at different levels in the rat hippocampus at different times after SE,suggesting that the activation of Caspase-3 may be involved in the excitotoxic effect induced by nitric oxide synthase （ NOS ） and apoptosis could be closely related to neuron injury.

关 键 词：癫痫持续状态 模型 动物 海马 Caspase蛋白 一氧化氮合酶 细胞凋亡 大鼠 Wistar 

分 类 号：R651.1[医药卫生—外科学] R-32[医药卫生—临床医学]