耐亚胺培南铜绿假单胞菌金属酶的筛选及基因型研究  

Screening and genotyping of metallo-β-Lactamase in imipenem-resistant Pseudomonas aeruginosa

作　　者：孙珊[1] 张莉萍[1] 

机构地区：[1]重庆医科大学附属第一医院检验科,重庆400016

出　　处：《中国微生态学杂志》2010年第11期1021-1023,共3页Chinese Journal of Microecology

摘　　要：目的研究重庆医科大学附属第一医院分离的29株耐亚胺培南铜绿假单胞菌中金属酶(Metallo-β-Lactamase,MBL)的基因型分布情况。方法用亚胺培南-EDTA纸片法筛选29株铜绿假单胞菌中产MBL的铜绿假单胞菌,用PCR扩增法检测29株菌中金属酶VIM和IMP基因。结果 29株耐亚胺培南的铜绿假单胞菌中,亚胺培南-EDTA纸片法筛选出MBL阳性菌株5株,阳性率为17%。PCR扩增出IMP基因型有4株,阳性率为14%,均为金属酶IMP-9型,未扩增出VIM基因。以PCR法结果为判定标准,亚胺培南-EDTA纸片法敏感性100%,特异性96%。结论 IMP-9型为该院分离的这29株耐亚胺培南的铜绿假单胞菌中主要MBL基因型。本实验中所用的亚胺培南-EDTA纸片法能简单有效的筛选出产MBL的铜绿假单胞菌。Objective To study the metallo-β-Lactamase in imipenem-resistant Pseudomonas aeruginosa （P. aeruginosa） isolated from our hospital. Method The imipenem-EDTA（IMP-EDTA） disc test was used to detect the metallo-β-Lactamase phenotypes and the PCR amplification method was used to detect the VIM and IMP genes. Result 5 strains of P. aeruginosa were positive for production of metallo-β-Laetarnase by IMP-EDTA disc test with the sensitivity of 100% and specificity of 96%. 4 strains ofP. aeruginosa were positive using the PCR method. The PCR products were analyzed by DNA sequencing, which showed that all the positive strains were IMP-9 type. Conclusion The P. aeruginosa producing IMP-9 metallo-β-Lactamase of the 29 strains P. aeruginosa in our hospital is prevalent in our hospital and the IMP- EDTA disc test can screen the metallo-β-Laetamase positive P. aeruginosa simply and effectively.

关 键 词：铜绿假单胞菌 金属酶 基因型 

分 类 号：R378.991[医药卫生—病原生物学]